On-demand optogenetic activation of human stem-cell-derived neurons
- The widespread application of human stem-cell-derived neurons for functional studies is impeded by complicated differentiation protocols, immaturity, and deficient optogene expression as stem cells frequently lose transgene expression over time. Here we report a simple but precise Cre-loxP-based strategy for generating conditional, and thereby stable, optogenetic human stem-cell lines. These cells can be easily and efficiently differentiated into functional neurons, and optogene expression can be triggered by administering Cre protein to the cultures. This conditional expression system may be applied to stem-cell-derived neurons whenever timed transgene expression could help to overcome silencing at the stem-cell level.
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| Author: | Simon Klapper, Evelyn J. Sauter, Anka Swiersy, Max A. E. Hyman, Christian BamannORCiDGND, Ernst BambergGND, Volker BusskampORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-449978 |
| DOI: | https://doi.org/10.1038/s41598-017-14827-6 |
| ISSN: | 2045-2322 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/29089561 |
| Parent Title (English): | Scientific reports |
| Publisher: | Macmillan Publishers Limited, part of Springer Nature |
| Place of publication: | [London] |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2017 |
| Date of first Publication: | 2017/10/31 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2017/12/14 |
| Tag: | Induced pluripotent stem cells; Optogenetics; Stem-cell differentiation |
| Volume: | 7 |
| Issue: | 1, Art. 14450 |
| Page Number: | 9 |
| First Page: | 1 |
| Last Page: | 9 |
| Note: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017 |
| HeBIS-PPN: | 426619544 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Angeschlossene und kooperierende Institutionen / MPI für Biophysik | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
