Kinetic fingerprinting links bacteria-phage interactions with emergent dynamics: Rapid depletion of klebsiella pneumoniae indicates phage synergy
- The specific temporal evolution of bacterial and phage population sizes, in particular bacterial depletion and the emergence of a resistant bacterial population, can be seen as a kinetic fingerprint that depends on the manifold interactions of the specific phage–host pair during the course of infection. We have elaborated such a kinetic fingerprint for a human urinary tract Klebsiella pneumoniae isolate and its phage vB_KpnP_Lessing by a modeling approach based on data from in vitro co-culture. We found a faster depletion of the initially sensitive bacterial population than expected from simple mass action kinetics. A possible explanation for the rapid decline of the bacterial population is a synergistic interaction of phages which can be a favorable feature for phage therapies. In addition to this interaction characteristic, analysis of the kinetic fingerprint of this bacteria and phage combination revealed several relevant aspects of their population dynamics: A reduction of the bacterial concentration can be achieved only at high multiplicity of infection whereas bacterial extinction is hardly accomplished. Furthermore the binding affinity of the phage to bacteria is identified as one of the most crucial parameters for the reduction of the bacterial population size. Thus, kinetic fingerprinting can be used to infer phage–host interactions and to explore emergent dynamics which facilitates a rational design of phage therapies.
Author: | Holger Lößner, Insea Schlattmeier, Marie Anders-Maurer, Isabelle Bekeredjian-Ding, Christine Rohde, Johannes Wittmann, Cornelia PokalyukGND, Oleg Krut, Christel KampORCiDGND |
---|---|
URN: | urn:nbn:de:hebis:30:3-542887 |
DOI: | https://doi.org/10.3390/antibiotics9070408 |
ISSN: | 2079-6382 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/32674401 |
Parent Title (English): | Antibiotics |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Year of Completion: | 2020 |
Date of first Publication: | 2020/07/14 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2020/07/22 |
Tag: | Klebsiella pneumoniae; emergence; kinetic fingerprint; mathematical modeling; phage; phage therapy; population dynamics; resistance; synergistic interaction |
Volume: | 9 |
Issue: | 7, Art. 408 |
Page Number: | 15 |
First Page: | 1 |
Last Page: | 15 |
Note: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
HeBIS-PPN: | 467547645 |
Institutes: | Informatik und Mathematik / Mathematik |
Informatik und Mathematik / Informatik | |
Medizin / Medizin | |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | ![]() |