Kinetic fingerprinting links bacteria-phage interactions with emergent dynamics: Rapid depletion of klebsiella pneumoniae indicates phage synergy

  • The specific temporal evolution of bacterial and phage population sizes, in particular bacterial depletion and the emergence of a resistant bacterial population, can be seen as a kinetic fingerprint that depends on the manifold interactions of the specific phage–host pair during the course of infection. We have elaborated such a kinetic fingerprint for a human urinary tract Klebsiella pneumoniae isolate and its phage vB_KpnP_Lessing by a modeling approach based on data from in vitro co-culture. We found a faster depletion of the initially sensitive bacterial population than expected from simple mass action kinetics. A possible explanation for the rapid decline of the bacterial population is a synergistic interaction of phages which can be a favorable feature for phage therapies. In addition to this interaction characteristic, analysis of the kinetic fingerprint of this bacteria and phage combination revealed several relevant aspects of their population dynamics: A reduction of the bacterial concentration can be achieved only at high multiplicity of infection whereas bacterial extinction is hardly accomplished. Furthermore the binding affinity of the phage to bacteria is identified as one of the most crucial parameters for the reduction of the bacterial population size. Thus, kinetic fingerprinting can be used to infer phage–host interactions and to explore emergent dynamics which facilitates a rational design of phage therapies.

Download full text files

Export metadata

Metadaten
Author:Holger Lößner, Insea Schlattmeier, Marie Anders-Maurer, Isabelle Bekeredjian-Ding, Christine Rohde, Johannes Wittmann, Cornelia Pokalyuk, Oleg Krut, Christel KampORCiDGND
URN:urn:nbn:de:hebis:30:3-542887
DOI:https://doi.org/10.3390/antibiotics9070408
ISSN:2079-6382
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/32674401
Parent Title (English):Antibiotics
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Completion:2020
Date of first Publication:2020/07/14
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/07/22
Tag:Klebsiella pneumoniae; emergence; kinetic fingerprint; mathematical modeling; phage; phage therapy; population dynamics; resistance; synergistic interaction
Volume:9
Issue:7, Art. 408
Page Number:15
First Page:1
Last Page:15
Note:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
HeBIS-PPN:467547645
Institutes:Informatik und Mathematik / Mathematik
Informatik und Mathematik / Informatik
Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0