Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO₂ reduction to formate

  • In times of global climate change and the fear of dwindling resources, we are facing different considerable challenges such as the replacement of fossil fuel–based energy carriers with the coincident maintenance of the increasing energy supply of our growing world population. Therefore, CO2 capturing and H2 storing solutions are urgently needed. In this study, we demonstrate the production of a functional and biotechnological interesting enzyme complex from acetogenic bacteria, the hydrogen-dependent CO2 reductase (HDCR), in the well-known model organism Escherichia coli. We identified the metabolic bottlenecks of the host organisms for the production of the HDCR enzyme complex. Here we show that the recombinant expression of a heterologous enzyme complex transforms E. coli into a whole-cell biocatalyst for hydrogen-driven CO2 reduction to formate without the need of any external co-factors or endogenous enzymes in the reaction process. This shifts the industrial platform organism E. coli more and more into the focus as biocatalyst for CO2-capturing and H2-storage. Key points: A functional HDCR enzyme complex was heterologously produced in E. coli; The metabolic bottlenecks for HDCR production were identified; HDCR enabled E. coli cell to capture and store H2 and CO2 in the form of formate.
Author:Felix Leo, Fabian M. SchwarzGND, Kai SchuchmannGND, Volker MüllerORCiD
Parent Title (English):Applied microbiology and biotechnology
Place of publication:Berlin; Heidelberg; New York
Document Type:Article
Date of Publication (online):2021/07/31
Date of first Publication:2021/07/31
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/10/04
Tag:Acetobacterium woodii; Biocatalysis; CO2 capture; Heterologous enzyme production; Hydrogen-dependent CO2 reductase
Page Number:12
First Page:5861
Last Page:5872
Open access funding enabled and organized by Projekt DEAL. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement number 741791).
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0