Gene-drive suppression of mosquito populations in large cages as a bridge between lab and field

  • CRISPR-based gene-drives targeting the gene doublesex in the malaria vector Anopheles gambiae effectively suppressed the reproductive capability of mosquito populations reared in small laboratory cages. To bridge the gap between laboratory and the field, this gene-drive technology must be challenged with vector ecology. Here we report the suppressive activity of the gene-drive in age-structured An. gambiae populations in large indoor cages that permit complex feeding and reproductive behaviours. The gene-drive element spreads rapidly through the populations, fully supresses the population within one year and without selecting for resistance to the gene drive. Approximate Bayesian computation allowed retrospective inference of life-history parameters from the large cages and a more accurate prediction of gene-drive behaviour under more ecologically-relevant settings. Generating data to bridge laboratory and field studies for invasive technologies is challenging. Our study represents a paradigm for the stepwise and sound development of vector control tools based on gene-drive.
Author:Andrew HammondORCiD, Paola PollegioniORCiD, Tania Persampieri, Ace NorthORCiD, Roxana MinuzORCiD, Alessandro Trusso, Alessandro Bucci, Kyros KyrouORCiD, Ioanna MorianouORCiD, Alekos SimoniORCiD, Tony NolanORCiD, Ruth MüllerORCiD, Andrea CrisantiORCiD
Parent Title (English):Nature Communications
Publisher:Nature Publishing Group UK
Place of publication:[London]
Document Type:Article
Date of Publication (online):2021/07/28
Date of first Publication:2021/07/28
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/07/11
Tag:Behavioural ecology; Environmental biotechnology; Population dynamics; Synthetic biology
Issue:art. 4589
Page Number:9
First Page:1
Last Page:9
Supported by a grant from the Bill & Melinda Gates Foundation and from the Open Philanthropy Project Fund, an advised fund of Silicon Valley Community Foundation.
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0