Untersuchungen über Komplexbildungen von Dehydrogenasen mit Coenzymanalogen

Investigations on complex formation of dehydrogenases with coenzyme analogues

  • The coenzyme analogue nicotinamide 5-iodouracil-dinucleotide was synthesized by condensation of the two mononucleotides with dicyclohexylcarbodiimide in aqueous pyridine. The enzymatic properties of this compound were compared with those of the nicotinamide-uracil-dinucleotide. Both coenzyme analogues reacted slowly when functioning as a hydrogen carrier in enzymatic tests. The properties were similar to those of nicotinamide-benzimidazole-dinucleotide. The difference spectrum between the intact coenzyme analogue and its mononucleotides showed that the intramolecular interaction between the functional and non-functional moiety was smaller than that in NAD. The interaction corresponded to that of nicotinamide-benzimidazole-dinucleotide. The fluorescence excitation spectrum did not show any energy transfer from the non-functional iodouracil to the dihydronicotinamide part of the analogue. Difference spectra between the coenzyme - enzymecomplex and the two isolated components indicated that the unfolded dihydrocoenzyme was bound to the active site of lactate- and alcohol-dehydrogenase, respectively. Furthermore, they showed aromatic interaction of the non-functional part with parts of the protein. Introduction of iodine into the nicotinamide-uracil-dinucleotide did not remarkably alter the behavior of the analogues. As the iodine is bound very strongly to the coenzyme analogue, it may be useful for X-Ray-investigations of the dehydrogenases.

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Author:Christoph Wilhelm Wönckhaus, Dietmar Scherr
Parent Title (German):Zeitschrift für Naturforschung, B
Publisher:Verlag der Zeitschrift für Naturforschung
Place of publication:Tübingen
Document Type:Article
Date of Publication (online):2014/06/02
Year of first Publication:1971
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/12/29
Page Number:7
First Page:106
Last Page:112
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0