Comparative toxicity assessment of in situ burn residues to initial and dispersed heavy fuel oil using zebrafish embryos as test organisms

  • In situ burning (ISB) is discussed to be one of the most suitable response strategies to combat oil spills in extreme conditions. After burning, a highly viscous and sticky residue is left and may over time pose a risk of exposing aquatic biota to toxic oil compounds. Scientific information about the impact of burn residues on the environment is scarce. In this context, a comprehensive ISB field experiment with approx. 1000L IFO 180 was conducted in a fjord in Greenland. The present study investigated the toxicity of collected ISB residues to early life stages of zebrafish (Danio rerio) as a model for potentially exposed pelagic organisms. The toxicity of ISB residues on zebrafish embryos was compared with the toxicity of the initial (unweathered) IFO 180 and chemically dispersed IFO 180. Morphological malformations, hatching success, swimming behavior, and biomarkers for exposure (CYP1A activity, AChE inhibition) were evaluated in order to cover the toxic response on different biological organization levels. Across all endpoints, ISB residues did not induce greater toxicity in zebrafish embryos compared with the initial oil. The application of a chemical dispersant increased the acute toxicity most likely due to a higher bioavailability of dissolved and particulate oil components. The results provide insight into the adverse effects of ISB residues on sensitive life stages of fish in comparison with chemical dispersant application.

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Author:Sarah JohannORCiDGND, Mira Goßen, Leonie Müller, Valentina Selja, Kim GustavsonORCiD, Janne Fritt-RasmussenORCiD, Susse Wegeberg, Tomasz Maciej CiesielskiORCiD, Bjørn Munro JenssenORCiD, Henner HollertORCiDGND, Thomas-Benjamin SeilerORCiDGND
Parent Title (English):Environmental science and pollution research
Place of publication:Berlin ; Heidelberg
Document Type:Article
Date of Publication (online):2020/12/03
Date of first Publication:2020/12/03
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/10/13
Tag:AChE; Chemical dispersant; EROD; Embryo toxicity; In situ burning; Swimming behavior
Page Number:16
First Page:16198
Last Page:16213
Open Access funding enabled and organized by Projekt DEAL. The work has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 679266.
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International