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Molecular keys to the Janthinobacterium and Duganella spp. interaction with the plant pathogen Fusarium graminearum
- Janthinobacterium and Duganella are well-known for their antifungal effects. Surprisingly, almost nothing is known on molecular aspects involved in the close bacterium-fungus interaction. To better understand this interaction, we established the genomes of 11 Janthinobacterium and Duganella isolates in combination with phylogenetic and functional analyses of all publicly available genomes. Thereby, we identified a core and pan genome of 1058 and 23,628 genes. All strains encoded secondary metabolite gene clusters and chitinases, both possibly involved in fungal growth suppression. All but one strain carried a single gene cluster involved in the biosynthesis of alpha-hydroxyketone-like autoinducer molecules, designated JAI-1. Genome-wide RNA-seq studies employing the background of two isolates and the corresponding JAI-1 deficient strains identified a set of 45 QS-regulated genes in both isolates. Most regulated genes are characterized by a conserved sequence motif within the promoter region. Among the most strongly regulated genes were secondary metabolite and type VI secretion system gene clusters. Most intriguing, co-incubation studies of J. sp. HH102 or its corresponding JAI-1 synthase deletion mutant with the plant pathogen Fusarium graminearum provided first evidence of a QS-dependent interaction with this pathogen.
Author: | Frederike Svenja Haack, Anja Poehlein, Cathrin Kröger, Christian A. Voigt, Meike PiepenbringORCiDGND, Helge Björn BodeORCiDGND, Rolf DanielORCiDGND, Wilhelm Schäfer, Wolfgang R. Streit |
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URN: | urn:nbn:de:hebis:30:3-436820 |
DOI: | https://doi.org/10.3389/fmicb.2016.01668 |
ISSN: | 1664-302X |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/27833590 |
Parent Title (English): | Frontiers in microbiology |
Publisher: | Frontiers Media |
Place of publication: | Lausanne |
Contributor(s): | Trevor Carlos Charles |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2017/06/26 |
Year of first Publication: | 2016 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2017/06/26 |
Tag: | CAI-1; Duganella; Fusarium graminearum; Janthinobacterium; bacterium-fungus interaction; quorum sensing; violacein |
Volume: | 7 |
Issue: | Art. 1668 |
Page Number: | 17 |
First Page: | 1 |
Last Page: | 17 |
Note: | Copyright © 2016 Haack, Poehlein, Kröger, Voigt, Piepenbring, Bode, Daniel, Schäfer and Streit. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
HeBIS-PPN: | 424953331 |
Institutes: | Biowissenschaften / Biowissenschaften |
Biowissenschaften / Institut für Ökologie, Evolution und Diversität | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |