Malte Bachmann, Katharina Horn, Ina Rudloff, Itamar Goren, Martin Holdener, Urs Christen, Nicole Darsow, Klaus-Peter Hunfeld, Ulrike Köhl, Peter Kind, Josef Pfeilschifter, Peter Kraiczy, Heiko Mühl
- If insufficiently treated, Lyme borreliosis can evolve into an inflammatory disorder affecting skin, joints, and the CNS. Early innate immunity may determine host responses targeting infection. Thus, we sought to characterize the immediate cytokine storm associated with exposure of PBMC to moderate levels of live Borrelia burgdorferi. Since Th17 cytokines are connected to host defense against extracellular bacteria, we focused on interleukin (IL)-17 and IL-22. Here, we report that, despite induction of inflammatory cytokines including IL-23, IL-17 remained barely detectable in response to B. burgdorferi. In contrast, T cell-dependent expression of IL-22 became evident within 10 h of exposure to the spirochetes. This dichotomy was unrelated to interferon-gamma but to a large part dependent on caspase-1 and IL-1 bioactivity derived from monocytes. In fact, IL-1beta as a single stimulus induced IL-22 but not IL-17. Neutrophils display antibacterial activity against B. burgdorferi, particularly when opsonized by antibodies. Since neutrophilic inflammation, indicative of IL-17 bioactivity, is scarcely observed in Erythema migrans, a manifestation of skin inflammation after infection, protective and antibacterial properties of IL-22 may close this gap and serve essential functions in the initial phase of spirochete infection.
MetadatenVerfasserangaben: | Malte BachmannGND, Katharina Horn, Ina RudloffGND, Itamar Goren, Martin Holdener, Urs ChristenORCiDGND, Nicole Darsow, Klaus-Peter HunfeldORCiD, Ulrike KöhlORCiDGND, Peter Kind, Josef PfeilschifterGND, Peter KraiczyGND, Heiko MühlORCiDGND |
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URN: | urn:nbn:de:hebis:30-83153 |
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DOI: | https://doi.org/10.1371/journal.ppat.1001144 |
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ISSN: | 1553-7374 |
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Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/20976193 |
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Titel des übergeordneten Werkes (Englisch): | PLoS pathogens |
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Verlag: | PLoS |
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Verlagsort: | Lawrence, Kan. |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Veröffentlichung (online): | 09.09.2010 |
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Datum der Erstveröffentlichung: | 09.09.2010 |
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Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Datum der Freischaltung: | 22.10.2010 |
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Jahrgang: | 6 |
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Ausgabe / Heft: | (10): e1001144 |
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Seitenzahl: | 16 |
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Erste Seite: | 1 |
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Letzte Seite: | 16 |
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Bemerkung: | Copyright: © 2010 Bachmann et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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HeBIS-PPN: | 229640028 |
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Institute: | Medizin / Medizin |
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| Fachübergreifende Einrichtungen / Zentrum für Arzneimittelforschung, Entwicklung und Sicherheit (ZAFES) |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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| Sammlung Biologie / Sondersammelgebiets-Volltexte |
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Lizenz (Deutsch): | Creative Commons - Namensnennung 3.0 |
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