Christian Haberlandt, Amin Derouiche, Alexandra Wyczynski, Julia Haseleu, Jörg Pohle, Khalad Karram, Jacqueline Trotter, Gerald Seifert, Michael Frotscher, Christian Steinhäuser, Ronald Jabs
- NG2 cells, the fourth type of glia in the mammalian CNS, receive synaptic input from neurons. The function of this innervation is unknown yet. Postsynaptic changes in intracellular Ca2+-concentration ([Ca2+]i) might be a possible consequence. We employed transgenic mice with fluorescently labeled NG2 cells to address this issue. To identify Ca2+-signaling pathways we combined patch-clamp recordings, Ca2+-imaging, mRNA-transcript analysis and focal pressure-application of various substances to identified NG2-cells in acute hippocampal slices. We show that activation of voltage-gated Ca2+-channels, Ca2+-permeable AMPA-receptors, and group I metabotropic glutamate-receptors provoke [Ca2+]i-elevations in NG2 cells. The Ca2+-influx is amplified by Ca2+-induced Ca2+-release. Minimal electrical stimulation of presynaptic neurons caused postsynaptic currents but no somatic [Ca2+]i elevations, suggesting that [Ca2+]i elevations in NG2 cells might be restricted to their processes. Local Ca2+-signaling might provoke transmitter release or changes in cell motility. To identify structural prerequisites for such a scenario, we used electron microscopy, immunostaining, mRNA-transcript analysis, and time lapse imaging. We found that NG2 cells form symmetric and asymmetric synapses with presynaptic neurons and show immunoreactivity for vesicular glutamate transporter 1. The processes are actin-based, contain ezrin but not glial filaments, microtubules or endoplasmic reticulum. Furthermore, we demonstrate that NG2 cell processes in situ are highly motile. Our findings demonstrate that gray matter NG2 cells are endowed with the cellular machinery for two-way communication with neighboring cells.
MetadatenAuthor: | Christian Haberlandt, Amin DerouicheORCiD, Alexandra Wyczynski, Julia Haseleu, Jörg Pohle, Khalad Karram, Jacqueline Trotter, Gerald Seifert, Michael Frotscher, Christian Steinhäuser, Ronald Jabs |
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URN: | urn:nbn:de:hebis:30-115047 |
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DOI: | https://doi.org/10.1371/journal.pone.0017575 |
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ISSN: | 1932-6203 |
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Parent Title (English): | PLoS One |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2011/03/24 |
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Date of first Publication: | 2011/03/24 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2011/09/08 |
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Volume: | 6 |
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Issue: | (3): e17575 |
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Note: | Copyright: © 2011 Haberlandt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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Source: | PLoS ONE 6(3): e17575. doi: 10.1371/journal.pone.0017575 |
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HeBIS-PPN: | 276336356 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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| Sammlung Biologie / Sondersammelgebiets-Volltexte |
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Licence (German): | Creative Commons - Namensnennung 3.0 |
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