Ralf Brandes, Anne Dueck, Stefan Engelhardt, Manuel Kaulich, Christian Kupatt, Maria Teresa De Angelis, Matthias Leisegang, Ferdinand Le Noble, Alessandra Moretti, Oliver J. Müller, Boris V. Skryabin, Thomas Thum, Wolfgang Wurst
- For a long time, gene editing had been a scientific concept, which was limited to a few applications. With recent developments, following the discovery of TALEN zinc-finger endonucleases and in particular the CRISPR/Cas system, gene editing has become a technique applicable in most laboratories. The current gain- and loss-of function models in basic science are revolutionary as they allow unbiased screens of unprecedented depth and complexity and rapid development of transgenic animals. Modifications of CRISPR/Cas have been developed to precisely interrogate epigenetic regulation or to visualize DNA complexes. Moreover, gene editing as a clinical treatment option is rapidly developing with first trials on the way. This article reviews the most recent progress in the field, covering expert opinions gathered during joint conferences on genome editing of the German Cardiac Society (DGK) and the German Center for Cardiovascular Research (DZHK). Particularly focusing on the translational aspect and the combination of cellular and animal applications, the authors aim to provide direction for the development of the field and the most frequent applications with their problems.
MetadatenAuthor: | Ralf BrandesORCiDGND, Anne DueckORCiD, Stefan EngelhardtORCiDGND, Manuel KaulichORCiD, Christian KupattORCiDGND, Maria Teresa De AngelisORCiD, Matthias LeisegangORCiDGND, Ferdinand Le NobleGND, Alessandra MorettiORCiD, Oliver J. MüllerORCiDGND, Boris V. SkryabinORCiD, Thomas ThumORCiDGND, Wolfgang WurstORCiD |
---|
URN: | urn:nbn:de:hebis:30:3-747393 |
---|
DOI: | https://doi.org/10.1007/s00395-020-00839-3 |
---|
ISSN: | 1435-1803 |
---|
Parent Title (English): | Basic research in cardiology |
---|
Publisher: | Steinkopff ; Springer |
---|
Place of publication: | [Darmstadt u.a.] ; Heidelberg |
---|
Document Type: | Article |
---|
Language: | English |
---|
Date of Publication (online): | 2021/01/15 |
---|
Date of first Publication: | 2021/01/15 |
---|
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
---|
Release Date: | 2023/10/25 |
---|
Tag: | Animal models; CRISPR/Cas; Genome editing |
---|
Volume: | 116 |
---|
Issue: | 1, art. 2 |
---|
Article Number: | 2 |
---|
Page Number: | 20 |
---|
First Page: | 1 |
---|
Last Page: | 20 |
---|
Note: | Open Access funding enabled and organized by Projekt DEAL. The initiative for this manuscript was supported by a grant from the DGK and the DZHK. |
---|
HeBIS-PPN: | 514462620 |
---|
Institutes: | Medizin |
---|
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
---|
Sammlungen: | Universitätspublikationen |
---|
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |
---|