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A comprehensive assessment of apigenin as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound

  • Apigenin (4′,5,7-trihydroxyflavone) (Api) is an important component of the human diet, being distributed in a wide number of fruits, vegetables and herbs with the most important sources being represented by chamomile, celery, celeriac and parsley. This study was designed for a comprehensive evaluation of Api as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound. In the set experimental conditions, Api presents antiproliferative activity against the A375 human melanoma cell line, a G2/M arrest of the cell cycle and cytotoxic events as revealed by the lactate dehydrogenase release. Caspase 3 activity was inversely proportional to the Api tested doses, namely 30 μM and 60 μM. Phenomena of early apoptosis, late apoptosis and necrosis following incubation with Api were detected by Annexin V-PI double staining. The flavone interfered with the mitochondrial respiration by modulating both glycolytic and mitochondrial pathways for ATP production. The metabolic activity of human dendritic cells (DCs) under LPS-activation was clearly attenuated by stimulation with high concentrations of Api. Il-6 and IL-10 secretion was almost completely blocked while TNF alpha secretion was reduced by about 60%. Api elicited antiangiogenic properties in a dose-dependent manner. Both concentrations of Api influenced tumour cell growth and migration, inducing a limited tumour area inside the application ring, associated with a low number of capillaries.

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Verfasserangaben:Alexandra Ghiţu, Anja Schwiebs, Heinfried H. RadekeORCiDGND, Stefana Avram, Istvan Zupko, Andrea Bor, Ioana Zinuca Pavel, Cristina Adriana Dehelean, Camelia Oprean, Florina Bojin, Claudia Farcas, Codruta Soica, Oana Duicu, Corina Danciu
URN:urn:nbn:de:hebis:30:3-500631
DOI:https://doi.org/10.3390/nu11040858
ISSN:2072-6643
Pubmed-Id:https://pubmed.ncbi.nlm.nih.gov/30995771
Titel des übergeordneten Werkes (Englisch):Nutrients
Verlag:MDPI
Verlagsort:Basel
Dokumentart:Wissenschaftlicher Artikel
Sprache:Englisch
Jahr der Fertigstellung:2019
Datum der Erstveröffentlichung:16.04.2019
Veröffentlichende Institution:Universitätsbibliothek Johann Christian Senckenberg
Datum der Freischaltung:02.05.2019
Freies Schlagwort / Tag:A375 human melanoma cell line; angiogenesis; apigenin; apoptosis; mitochondrial bioenergetics and glycolysis; proliferation
Jahrgang:11
Ausgabe / Heft:4, Art. 858
Seitenzahl:19
Erste Seite:1
Letzte Seite:19
Bemerkung:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
HeBIS-PPN:450826961
Institute:Medizin / Medizin
DDC-Klassifikation:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Lizenz (Deutsch):License LogoCreative Commons - Namensnennung 4.0