Application of pseudotyped virus particles to monitor Ebola virus and SARS-CoV-2 viral entry in human cell lines
- Experimental work on highly pathogenic viruses such as Ebola virus (EBOV) and severe acute respiratory syndrome coronavirus-2 requires high-level biosafety facilities. Here, we provide a detailed step-by-step protocol which details the production and application of replication-incompetent murine leukemia virus-based pseudotyped particles to monitor and quantify the viral entry efficiency in human cell lines under biosafety level-2 conditions. We describe the use of viral particles encoding luciferase gene and the quantification of transduction efficiency by measuring luciferase activity.
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| Author: | Madeleine EichlerGND, Ebru AksiORCiD, Josef PfeilschifterGND, Gergely ImreORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-779423 |
| DOI: | https://doi.org/10.1016/j.xpro.2021.100818 |
| ISSN: | 2666-1667 |
| Parent Title (English): | STAR Protocols |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/08/14 |
| Date of first Publication: | 2021/08/27 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/10/23 |
| Tag: | Cell culture; Cell-based Assays; Immunology; Microbiology; Molecular Biology |
| Volume: | 2 |
| Issue: | 4, art. 100818 |
| Article Number: | 100818 |
| Page Number: | 14 |
| HeBIS-PPN: | 516756370 |
| Institutes: | Medizin |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
