Christine Herzog, Martina Schmitz, Bodo Levkau, Ilka Herrgott, Jan Mersmann, Jan Larmann, Kai Johanning, Michael Winterhalter, Jerold Chun, Frank Ulrich Müller, Frank Echtermeyer, Reinhard Hildebrand, Gregor Theilmeier
- HDL, through sphingosine-1-phosphate (S1P), exerts direct cardioprotective effects on ischemic myocardium. It remains unclear whether other HDL-associated sphingophospholipids have similar effects. We therefore examined if HDL-associated sphingosylphosphorylcholine (SPC) reduces infarct size in a mouse model of transient myocardial ischemia/reperfusion. Intravenously administered SPC dose-dependently reduced infarct size after 30 minutes of myocardial ischemia and 24 hours reperfusion compared to controls. Infarct size was also reduced by postischemic, therapeutical administration of SPC. Immunohistochemistry revealed reduced polymorphonuclear neutrophil recruitment to the infarcted area after SPC treatment, and apoptosis was attenuated as measured by TUNEL. In vitro, SPC inhibited leukocyte adhesion to TNFα-activated endothelial cells and protected rat neonatal cardiomyocytes from apoptosis. S1P3 was identified as the lysophospholipid receptor mediating the cardioprotection by SPC, since its effect was completely absent in S1P3-deficient mice. We conclude that HDL-associated SPC directly protects against myocardial reperfusion injury in vivo via the S1P3 receptor.
MetadatenAuthor: | Christine Herzog, Martina Schmitz, Bodo Levkau, Ilka Herrgott, Jan MersmannORCiDGND, Jan Larmann, Kai Johanning, Michael Winterhalter, Jerold ChunORCiDGND, Frank Ulrich Müller, Frank Echtermeyer, Reinhard Hildebrand, Gregor Theilmeier |
---|
URN: | urn:nbn:de:hebis:30-89145 |
---|
DOI: | https://doi.org/10.1155/2010/425191 |
---|
ISSN: | 1466-1861 |
---|
ISSN: | 0962-9351 |
---|
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/21274265 |
---|
Parent Title (English): | Mediators of inflammation |
---|
Publisher: | Hindawi Publishing Corp. |
---|
Place of publication: | Sylvania, Ohio |
---|
Contributor(s): | Andrew Parker |
---|
Document Type: | Article |
---|
Language: | English |
---|
Date of Publication (online): | 2011/01/12 |
---|
Date of first Publication: | 2011/01/03 |
---|
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
---|
Release Date: | 2011/01/12 |
---|
Volume: | 2010 |
---|
Issue: | Art. 425191 |
---|
Page Number: | 7 |
---|
First Page: | 1 |
---|
Last Page: | 7 |
---|
Note: | Copyright © 2010 Christine Herzog et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
---|
Source: | Mediators of Inflammation ; vol. 2010 (2010) ; article ID: 425191 ; 7 pages ; doi: 10.1155/2010/425191 |
---|
HeBIS-PPN: | 231036396 |
---|
Institutes: | Medizin / Medizin |
---|
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
---|
Sammlungen: | Universitätspublikationen |
---|
Licence (German): | Creative Commons - Namensnennung 3.0 |
---|