Über Desoxyribonucleinsäure-Molekeln in Protein-Mischfilmen
- A nucleoprotein of a vitrous consistency was extracted from the gonads of the coalfish (Gadus virens). The preparation of deoxyribonucleic acid (DNA) from this nucleoprotein and from staphylococci is described. Both of these different kinds of DNA have been mixed with bovine serum albumin or cytochrom c respectively to produce solutions which subsequently were spread onto the Langmuir trough under defined conditions. After transfer of aliquots from the surface monolayers to carbon support films the preparations were examined with the electron microscope. The micrographs show threads of various lengths, partly stretched, partly folded in loops, consisting of DNA molecules embedded in a protein envelope. Measurements and calculations of 5900 particles of the complex of Gadus virens-DNA-Albumin, with relatively short threads show a distribution of discontinuous character. If length is plotted against number then it occurs that there are maxima of different lengths of threads. The abscissae of these maxima obey the ratio 1 : 2 : 4 : 8. This holds for longer threads too the maxima of which, however, have smaller ordinate values.
Author: | Albrecht KleinschmidtGND, H. Rüter, W. Hellmann, Rudolf K. Zahn, Annesuse Docter, E. Zimmermann, Horst Rübner, A. M. Al Ajwady |
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URN: | urn:nbn:de:hebis:30:3-698452 |
DOI: | https://doi.org/10.1515/znb-1959-1206 |
ISSN: | 1865-7117 |
ISSN: | 0932-0776 |
Parent Title (German): | Zeitschrift für Naturforschung, B |
Publisher: | Verlag der Zeitschrift für Naturforschung |
Place of publication: | Tübingen |
Document Type: | Article |
Language: | German |
Date of Publication (online): | 2014/06/02 |
Year of first Publication: | 1959 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/02/07 |
Volume: | 14.1959 |
Issue: | 12 |
Page Number: | 12 |
First Page: | 770 |
Last Page: | 779 |
Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |