Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores
- We demonstrate bleaching-independent STED microscopy using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging in fixed and living cells. Foremost, we achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multi-color and live cell STED microscopy with up to 100 min acquisition time.
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| Author: | Christoph Klaus SpahnORCiDGND, Jonathan B. GrimmORCiD, Luke D. LavisORCiD, Marko LampeORCiDGND, Mike HeilemannORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-725009 |
| DOI: | https://doi.org/10.1101/420638 |
| Parent Title (English): | bioRxiv |
| Document Type: | Preprint |
| Language: | English |
| Date of Publication (online): | 2018/09/18 |
| Date of first Publication: | 2018/09/18 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/07/26 |
| Issue: | 420638 |
| Page Number: | 7 |
| HeBIS-PPN: | 510563694 |
| Institutes: | Biochemie, Chemie und Pharmazie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
