Protein C preserves microcirculation in a model of neonatal septic shock
- Objectives: Sepsis remains a disease with a high mortality in neonates. Microcirculatory impairment plays a pivotal role in the development of multiorgan failure in septic newborns. The hemodynamic effects of recombinant activated protein C (rhAPC) were tested in an animal model of neonatal septic shock focusing on intestinal microcirculation. Materials and methods: Endotoxic shock was triggered by intravenous application of Escherichia coli lipopolysaccarides in newborn piglets. Thereafter, five animals received a continuous infusion of 24 µg/kg/h rhAPC, and five received vehicle for control. Over the course of three hours, intestinal microcirculation was assessed by intravital microscopy every 30 min. Macrocirculation and blood counts were monitored simultaneously. Results: After a short hypotensive period in all animals, the arterial blood pressure returned to baseline in the rhAPC-treated piglets, whereas the hypotension became increasingly severe in the controls. By 90 min, mean blood pressure in the controls was significantly lower than in the treatment group. Similar observations were made regaring microcirculation. After an early impairment in all study animals, functional capillary density and intestinal microcirculatory red blood cell velocity and red blood cell flow recovered in the rhAPC group, but deteriorated further in the control piglets. Conclusion: Recombinant activated protein C protects macro- and microcirculation from endotoxic shock.
Author: | Doris Fischer, Marcel Friedrich Nold, Claudia A. Nold-Petry, Antonio Furlan, Alex Veldman |
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URN: | urn:nbn:de:hebis:30:3-255408 |
DOI: | https://doi.org/10.2147/VHRM.S5863 |
ISSN: | 1176-6344 |
ISSN: | 1178-2048 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/19774219 |
Parent Title (English): | Vascular health and risk management |
Publisher: | Dove Medical Press |
Place of publication: | Albany, Auckland |
Document Type: | Article |
Language: | English |
Year of Completion: | 2009 |
Date of first Publication: | 2009/09/09 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2012/07/16 |
Tag: | intravital microscopy; microcirculation; neonatal septic shock; protein C |
Volume: | 5 |
Page Number: | 7 |
First Page: | 775 |
Last Page: | 781 |
Note: | This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms. |
HeBIS-PPN: | 357824725 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 |