Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1
- The respiratory chain in the inner mitochondrial membrane contains three large multi-enzyme complexes that together establish the proton gradient for ATP synthesis, and assemble into a supercomplex. A 19-Å 3D map of the 1.7-MDa amphipol-solubilized supercomplex I1III2IV1 from bovine heart obtained by single-particle electron cryo-microscopy reveals an amphipol belt replacing the membrane lipid bilayer. A precise fit of the X-ray structures of complex I, the complex III dimer, and monomeric complex IV indicates distances of 13 nm between the ubiquinol-binding sites of complexes I and III, and of 10–11 nm between the cytochrome c binding sites of complexes III and IV. The arrangement of respiratory chain complexes suggests two possible pathways for efficient electron transfer through the supercomplex, of which the shorter branch through the complex III monomer proximal to complex I may be preferred.
| Author: | Thorsten Althoff, Deryck J. MillsORCiD, Jean-Luc Popot, Werner KühlbrandtORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-259298 |
| DOI: | https://doi.org/10.1038/emboj.2011.324 |
| ISSN: | 0261-4189 |
| ISSN: | 1460-2075 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/21909073 |
| Parent Title (English): | The EMBO journal |
| Publisher: | Nature Publ. Group |
| Place of publication: | London |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2012/08/30 |
| Date of first Publication: | 2011/09/09 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2012/08/30 |
| Tag: | amphipols; electron cryo-microscopy; membrane protein complex; respiratory chain; single-particle averaging |
| Volume: | 30 |
| Page Number: | 13 |
| First Page: | 4652 |
| Last Page: | 4664 |
| HeBIS-PPN: | 358058597 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Sammlung Biologie / Sondersammelgebiets-Volltexte | |
| Licence (German): |  Creative Commons - Namensnennung-Keine kommerzielle Nutzung-Weitergabe unter gleichen Bedingungen |
