miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
- This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3βP) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3βP and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.
| Author: | Jose Luis ViñasORCiD, Marina Ventayol, Bernhard BrüneORCiD, Michaela JungORCiD, Anna Sola, Felip Pi, Chrysoula Mastora, Georgina HotterORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-297474 |
| DOI: | https://doi.org/10.1371/journal.pone.0060937 |
| ISSN: | 1932-6203 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/23593353 |
| Parent Title (English): | PLoS One |
| Publisher: | PLoS [u.a.] |
| Place of publication: | Lawrence, Kan. |
| Document Type: | Article |
| Language: | English |
| Date of first Publication: | 2013/04/10 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2013/04/11 |
| Volume: | 8 |
| Issue: | (4): e60937 |
| Page Number: | 8 |
| Note: | Copyright: © 2013 Viñas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| HeBIS-PPN: | 33700076X |
| Institutes: | Medizin / Medizin |
| Fachübergreifende Einrichtungen / Zentrum für Arzneimittelforschung, Entwicklung und Sicherheit | |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 3.0 |
