miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation

  • This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3βP) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3βP and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.

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Author:Jose Luis ViñasORCiD, Marina Ventayol, Bernhard BrüneORCiD, Michaela JungORCiD, Anna Sola, Felip Pi, Chrysoula Mastora, Georgina HotterORCiD
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/23593353
Parent Title (English):PLoS One
Publisher:PLoS [u.a.]
Place of publication:Lawrence, Kan.
Document Type:Article
Date of first Publication:2013/04/10
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2013/04/11
Issue:(4): e60937
Page Number:8
Copyright: © 2013 Viñas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Institutes:Medizin / Medizin
Fachübergreifende Einrichtungen / Zentrum für Arzneimittelforschung, Entwicklung und Sicherheit
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 3.0