Characterization of microsatellite loci in the lichen-forming fungus cetraria aculeata (parmeliaceae, ascomycota)

  • Premise of the study: Polymorphic microsatellite markers were developed for the lichen species Cetraria aculeata (Parmeliaceae) to study fine-scale population diversity and phylogeographic structure. Methods and Results: Using Illumina HiSeq and MiSeq, 15 fungus-specific microsatellite markers were developed and tested on 81 specimens from four populations from Spain. The number of alleles ranged from four to 13 alleles per locus with a mean of 7.9, and average gene diversities varied from 0.40 to 0.73 over four populations. The amplification rates of 10 markers (CA01– CA10) in populations of C. aculeata exceeded 85%. The markers also amplified across a range of closely related species, except for locus CA05, which did not amplify in C. australiensis and C. "panamericana," and locus CA10 which did not amplify in C. australiensis. Conclusions: The identified microsatellite markers will be used to study the genetic diversity and phylogeographic structure in populations of C. aculeata in western Eurasia.

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Author:Tetiana Lutsak, Fernando Fernández Mendoza, Bastian Greshake Tzovaras, Francesco Dal Grande, Ingo EbersbergerORCiDGND, Sieglinde Ott, Christian Printzen
URN:urn:nbn:de:hebis:30:3-422651
URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033364
DOI:https://doi.org/10.3732/apps.1600047
ISSN:2168-0450
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/27672520
Parent Title (English):Applications in plant sciences
Publisher:BioOne
Place of publication:Washington, DC
Document Type:Article
Language:English
Date of Publication (online):2016/12/01
Date of first Publication:2016/09/19
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2016/12/01
Tag:Cetraria aculeata; Parmeliaceae; lichen; microsatellites
Volume:4
Issue:(9):1600047
Page Number:5
First Page:1
Last Page:4
Note:
© 2016 Lutsak et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
HeBIS-PPN:428668976
Institutes:Biowissenschaften / Biowissenschaften
Angeschlossene und kooperierende Institutionen / Senckenbergische Naturforschende Gesellschaft
Fachübergreifende Einrichtungen / Biodiversität und Klima Forschungszentrum (BiK-F)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Keine kommerzielle Nutzung-Weitergabe unter gleichen Bedingungen