Transepithelial transport of putrescine across monolayers of the human intestinal epithelial cell line, Caco-2
- Aim: To study the transepithelial transport characteristics of the polyamine putrescine in human intestinal Caco-2 cell monolayers to elucidate the mechanisms of the putrescine intestinal absorption. Methods: The transepithelial transport and the cellular accumulation of putrescine was measured using Caco-2 cell monolayers grown on permeable filters. Results: Transepithelial transport of putrescine in physiological concentrations ( > 0.5 mM) from the apical to basolateral side was linear. Intracellular accumulation of putrescine was higher in confluent than in fully differentiated Caco-2 cells, but still negligible (less than 0.5%) of the overall transport across the monolayers in apical to basolateral direction.EGF enhanced putrescine accumulation in Caco-2 cells by four fold, as well as putrescine conversion to spermidine and spermine by enhancing the activity of S adenosylmethionine decarboxylase. However, EGF did not have any significant influence on putrescine flux across the Caco- 2 cell monolayers. Excretion of putrescine from Caco-2 cells into the basolateral medium did not exceed 50 picomoles, while putrescine passive flux from the apical to the basolateral chamber, contributed hundreds of micromoles polyamines to the basolateral chamber. Conclusion: Transepithelial transport of putrescine across Caco-2 cell monolayers occurs in passive diffusion, and is not influenced when epithelial cells are stimulated to proliferate by a potent mitogen such as EGF.
Author: | Vladan Milovic, Lyudmila Turchanowa, Jürgen SteinORCiDGND, Wolfgang F. CasparyGND |
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URN: | urn:nbn:de:hebis:30:3-456206 |
DOI: | https://doi.org/10.3748/wjg.v7.i2.193 |
ISSN: | 2219-2840 |
ISSN: | 1007-9327 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/11819759 |
Parent Title (English): | World journal of gastroenterology |
Publisher: | WJG Press |
Place of publication: | Beijing |
Document Type: | Article |
Language: | English |
Year of Completion: | 2001 |
Date of first Publication: | 2001/04/15 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2018/02/06 |
Tag: | Caco 2 cells/metabolism; biological transport; polyamines/metabolism; putrescine/metabolism |
Volume: | 7 |
Issue: | 2 |
Page Number: | 5 |
First Page: | 193 |
Last Page: | 197 |
Note: | This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
HeBIS-PPN: | 432160760 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (English): | Creative Commons - Namensnennung-Nicht kommerziell 4.0 |