Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1

  • Ribosome recycling orchestrated by ABCE1 is a fundamental process in protein translation and mRNA surveillance, connecting termination with initiation. Beyond the plenitude of well-studied translational GTPases, ABCE1 is the only essential factor energized by ATP, delivering the energy for ribosome splitting via two nucleotide-binding sites by a yet unknown mechanism. Here, we define how allosterically coupled ATP binding and hydrolysis events in ABCE1 empower ribosome recycling. ATP occlusion in the low-turnover control site II promotes formation of the pre-splitting complex and facilitates ATP engagement in the high-turnover site I, which in turn drives the structural reorganization required for ribosome splitting. ATP hydrolysis and ensuing release of ABCE1 from the small subunit terminate the post-splitting complex. Thus, ABCE1 runs through an allosterically coupled cycle of closure and opening at both sites, consistent with a processive clamp model. This study delineates the inner mechanics of ABCE1 and reveals why various ABCE1 mutants lead to defects in cell homeostasis, growth, and differentiation.

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Author:Elina Nürenberg-GoloubORCiDGND, Holger Heinemann, Milan Gerovac, Robert TampéORCiDGND
URN:urn:nbn:de:hebis:30:3-471082
DOI:https://doi.org/10.26508/lsa.201800095
ISSN:2575-1077
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30198020
Parent Title (English):Life science alliance
Publisher:Life Science Alliance, LLC
Place of publication:[Woodbury, NY]
Document Type:Article
Language:English
Year of Completion:2018
Date of first Publication:2018/06/14
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/09/20
Tag:ABC proteins; ATPases; conformational dynamics; mRNA translation; macromolecular complexes; molecular motors; ribosome quality control
Volume:1
Issue:3, e201800095
Page Number:12
First Page:1
Last Page:12
Note:
Copyright & Usage : © 2018 Nürenberg-Goloub et al. https://creativecommons.org/licenses/by/4.0/
This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
HeBIS-PPN:43784837X
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0