Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1
- Ribosome recycling orchestrated by ABCE1 is a fundamental process in protein translation and mRNA surveillance, connecting termination with initiation. Beyond the plenitude of well-studied translational GTPases, ABCE1 is the only essential factor energized by ATP, delivering the energy for ribosome splitting via two nucleotide-binding sites by a yet unknown mechanism. Here, we define how allosterically coupled ATP binding and hydrolysis events in ABCE1 empower ribosome recycling. ATP occlusion in the low-turnover control site II promotes formation of the pre-splitting complex and facilitates ATP engagement in the high-turnover site I, which in turn drives the structural reorganization required for ribosome splitting. ATP hydrolysis and ensuing release of ABCE1 from the small subunit terminate the post-splitting complex. Thus, ABCE1 runs through an allosterically coupled cycle of closure and opening at both sites, consistent with a processive clamp model. This study delineates the inner mechanics of ABCE1 and reveals why various ABCE1 mutants lead to defects in cell homeostasis, growth, and differentiation.
Author: | Elina Nürenberg-GoloubORCiDGND, Holger HeinemannORCiDGND, Milan Gerovac, Robert TampéORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-471082 |
DOI: | https://doi.org/10.26508/lsa.201800095 |
ISSN: | 2575-1077 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/30198020 |
Parent Title (English): | Life science alliance |
Publisher: | Life Science Alliance, LLC |
Place of publication: | [Woodbury, NY] |
Document Type: | Article |
Language: | English |
Year of Completion: | 2018 |
Date of first Publication: | 2018/06/14 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2018/09/20 |
Tag: | ABC proteins; ATPases; conformational dynamics; mRNA translation; macromolecular complexes; molecular motors; ribosome quality control |
Volume: | 1 |
Issue: | 3, e201800095 |
Page Number: | 12 |
First Page: | 1 |
Last Page: | 12 |
Note: | Copyright & Usage : © 2018 Nürenberg-Goloub et al. https://creativecommons.org/licenses/by/4.0/ This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
HeBIS-PPN: | 43784837X |
Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |