High-resolution small RNA structures from exact nuclear Overhauser enhancement measurements without additional restraints

  • RNA not only translates the genetic code into proteins, but also carries out important cellular functions. Understanding such functions requires knowledge of the structure and dynamics at atomic resolution. Almost half of the published RNA structures have been solved by nuclear magnetic resonance (NMR). However, as a result of severe resonance overlap and low proton density, high-resolution RNA structures are rarely obtained from nuclear Overhauser enhancement (NOE) data alone. Instead, additional semi-empirical restraints and labor-intensive techniques are required for structural averages, while there are only a few experimentally derived ensembles representing dynamics. Here we show that our exact NOE (eNOE) based structure determination protocol is able to define a 14-mer UUCG tetraloop structure at high resolution without other restraints. Additionally, we use eNOEs to calculate a two-state structure, which samples its conformational space. The protocol may open an avenue to obtain high-resolution structures of small RNA of unprecedented accuracy with moderate experimental efforts.
Author:Parker J. Nichols, Morkos A. Henen, Alexandra Born, Dean Strotz, Peter GüntertORCiDGND, Beat VögeliORCiD
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30271943
Parent Title (English):Communications biology
Publisher:Springer Nature
Place of publication:London
Document Type:Article
Year of Completion:2018
Date of first Publication:2018/06/07
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/11/29
Tag:Molecular conformation; Solution-state NMR
Issue:Art. 61
Page Number:11
First Page:1
Last Page:11
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Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Licence (German):License LogoCreative Commons - Namensnennung 4.0