Cerebrospinal fluid microRNAs are potential biomarkers of temporal lobe epilepsy and status epilepticus

  • There is a need for diagnostic biomarkers of epilepsy and status epilepticus to support clinical examination, electroencephalography and neuroimaging. Extracellular microRNAs may be potentially ideal biomarkers since some are expressed uniquely within specific brain regions and cell types. Cerebrospinal fluid offers a source of microRNA biomarkers with the advantage of being in close contact with the target tissue and sites of pathology. Here we profiled microRNA levels in cerebrospinal fluid from patients with temporal lobe epilepsy or status epilepticus, and compared findings to matched controls. Differential expression of 20 microRNAs was detected between patient groups and controls. A validation phase included an expanded cohort and samples from patients with other neurological diseases. This identified lower levels of miR-19b in temporal lobe epilepsy compared to controls, status epilepticus and other neurological diseases. Levels of miR-451a were higher in status epilepticus compared to other groups whereas miR-21-5p differed in status epilepticus compared to temporal lobe epilepsy but not to other neurological diseases. Targets of these microRNAs include proteins regulating neuronal death, tissue remodelling, gliosis and inflammation. The present study indicates cerebrospinal fluid contains microRNAs that can support differential diagnosis of temporal lobe epilepsy and status epilepticus from other neurological and non-neurological diseases.

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Author:Rana Raoof, Eva M. Jimenez-Mateos, Sebastian BauerORCiD, Björn Tackenberg, Felix RosenowORCiDGND, Johannes Lang, Müjgan Dogan Onugoren, Hajo Hamer, Tessa Huchtemann, Péter Körtvélyessy, Niamh M. C. Connolly, Shona Pfeiffer, Jochen H. M. Prehn, Michael A. Farrell, Donncha O’Brien, David C. Henshall, Catherine Mooney
URN:urn:nbn:de:hebis:30:3-486805
DOI:https://doi.org/10.1038/s41598-017-02969-6
ISSN:2045-2322
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/28607431
Parent Title (English):Scientific reports
Publisher:Macmillan Publishers Limited, part of Springer Nature
Place of publication:[London]
Document Type:Article
Language:English
Year of Completion:2017
Date of first Publication:2017/06/12
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/01/17
Tag:Diagnostic markers; Epilepsy
Volume:7
Issue:1, Art. 3328
Page Number:17
First Page:1
Last Page:17
Note:
Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
HeBIS-PPN:446224421
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0