Membrane potential (Vmem) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation

  • Background: Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or Vmem. Vmem plays a key role in numerous inter- and intracellular functions that regulate cell behaviors like proliferation, differentiation and migration, all playing a critical role in embryonic development, healing, and regeneration. Methods: With the goal of analyzing the changes in Vmem during cell proliferation and differentiation, here we used direct current electrical stimulation (EStim) to promote cell proliferation and differentiation and simultaneously tracked the corresponding changes in Vmem in adipose derived mesenchymal stem cells (AT-MSC). Results: We found that EStim caused increased AT-MSC proliferation that corresponded to Vmem depolarization and increased osteogenic differentiation that corresponded to Vmem hyperpolarization. Taken together, this shows that Vmem changes associated with EStim induced cell proliferation and differentiation can be accurately tracked during these important cell functions. Using this tool to monitor Vmem changes associated with these important cell behaviors we hope to learn more about how these electrochemical cues regulate cell function with the ultimate goal of developing new EStim based treatments capable of controlling healing and regeneration.
Metadaten
Author:Mit Balvantray BhavsarORCiD, Gloria Cato, Alexander Hauschild, Liudmila LeppikORCiD, Karla Mychellyne Costa OliveiraORCiD, Maria José Eischen-Loges, John Howard BarkerORCiD
URN:urn:nbn:de:hebis:30:3-490677
DOI:https://doi.org/10.7717/peerj.6341
ISSN:2167-8359
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/30775170
Parent Title (English):PeerJ
Publisher:PeerJ, Inc.
Place of publication:London [u. a.]
Contributor(s):Shi-Cong Tao
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/02/08
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/03/21
Tag:Cell proliferation; Electrical stimulation; Membrane potential; Mesenchymal stem cells; Osteogenic differentiation; Vmem
Volume:7
Issue:e6341
Page Number:13
First Page:1
Last Page:13
Note:
Distributed under Creative Commons CC-BY 4.0
HeBIS-PPN:448057107
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0