A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds
- We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.
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| Author: | Frederic StroblORCiDGND, Ernst H. K. StelzerORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-627795 |
| DOI: | https://doi.org/10.1038/s41598-021-94288-0 |
| ISSN: | 2045-2322 |
| Parent Title (English): | Scientific reports |
| Publisher: | Macmillan Publishers Limited, part of Springer Nature |
| Place of publication: | [London] |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/07/28 |
| Date of first Publication: | 2021/07/28 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2022/05/11 |
| Tag: | Entomology; Genetic vectors; Genotyping and haplotyping; Transgenic organisms |
| Volume: | 11 |
| Issue: | art. 15325 |
| Page Number: | 9 |
| First Page: | 1 |
| Last Page: | 9 |
| Note: | Open Access funding enabled and organized by Projekt DEAL. The research was funded by the Cluster of Excellence Frankfurt for Macromolecular Complexes (CEF-MC, EXC 115) granted to the Buchmann Institute for Molecular Life Sciences (BMLS) at the Goethe-Universität Frankfurt am Main by the Deutsche Forschungsgemeinschaft (DFG) and Joachim Herz Stiftung, Addon Fellowship 2019. |
| HeBIS-PPN: | 49640718X |
| Institutes: | Biowissenschaften |
| Fachübergreifende Einrichtungen / Buchmann Institut für Molekulare Lebenswissenschaften (BMLS) | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
