A Na+ A1AO ATP synthase with a V-type c subunit in a mesophilic bacterium

  • A1AO ATP synthases with a V-type c subunit have only been found in hyperthermophilic archaea which makes bioenergetic analyses impossible due to the instability of liposomes at high temperatures. A search for a potential archaeal A1AO ATP synthase with a V-type c subunit in a mesophilic organism revealed an A1AO ATP synthase cluster in the anaerobic, acetogenic bacterium Eubacterium limosum KIST612. The enzyme was purified to apparent homogeneity from cells grown on methanol to a specific activity of 1.2 U·mg−1 with a yield of 12%. The enzyme contained subunits A, B, C, D, E, F, H, a, and c. Subunit c is predicted to be a typical V-type c subunit with only one ion (Na+)-binding site. Indeed, ATP hydrolysis was strictly Na+-dependent. N,N′-dicyclohexylcarbodiimide (DCCD) inhibited ATP hydrolysis, but inhibition was relieved by addition of Na+. Na+ was shown directly to abolish binding of the fluorescence DCCD derivative, NCD-4, to subunit c, demonstrating a competition of Na+ and DCCD/NCD-4 for a common binding site. After incorporation of the A1AO ATP synthase into liposomes, ATP-dependent primary transport of 22Na+ as well as ΔµNa+-driven ATP synthesis could be demonstrated. The Na+ A1AO ATP synthase from E. limosum is the first ATP synthase with a V-type c subunit from a mesophilic organism. This will enable future bioenergetic analysis of these unique ATP synthases.

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Metadaten
Author:Dennis Litty, Volker MüllerORCiD
URN:urn:nbn:de:hebis:30:3-638257
DOI:https://doi.org/10.1111/febs.15193
ISSN:1742-4658
Parent Title (English):The FEBS journal
Publisher:Wiley-Blackwell
Place of publication:Oxford [u.a.]
Document Type:Article
Language:English
Date of Publication (online):2019/12/26
Date of first Publication:2019/12/26
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/03/08
Tag:Eubacterium; Na+ transport; acetogen; bioenergetics
Volume:287
Issue:14
Page Number:12
First Page:3012
Last Page:3023
Note:
Financial support by the Deutsche Forschungsgemeinschaft via SFB807 is gratefully acknowledged.
HeBIS-PPN:494118016
Institutes:Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0