Formate metabolism in the acetogenic bacterium Acetobacterium woodii

  • Acetogenic bacteria are already established as biocatalysts for production of high-value compounds from C1 substrates such as H2 + CO2 or CO. However, little is known about the physiology, biochemistry and bioenergetics of acetogenesis from formate, an interesting feedstock for biorefineries. Here, we analysed formate metabolism in the model acetogen Acetobacterium woodii. Cells grew optimally on 200 mM formate to an optical density of 0.6. Formate was exclusively converted to acetate (and CO2) with a ratio of 4.4:1. Transcriptome analyses revealed genes/enzymes involved in formate metabolism. Strikingly, A. woodii has two genes potentially encoding a formyl-THF synthetase, fhs1 and fhs2. fhs2 forms an operon with a gene encoding a potential formate transporter, fdhC. Deletion of fhs2/fdhC led to a reduced growth rate, formate consumption and optical densities. Acetogenesis from H2 + CO2 was accompanied by transient formate production; strikingly, formate reutilization was completely abolished in the Δfhs2/fdhC mutant. Take together, our studies gave the first detailed insights into the formatotrophic lifestyle of A. woodii.
Author:Jimyung MoonORCiD, Judith Dönig, Sina Kramer, Anja Poehlein, Rolf DanielORCiDGND, Volker MüllerORCiD
Parent Title (English):Environmental microbiology
Place of publication:Oxford [u.a.]
Document Type:Article
Date of Publication (online):2021/05/14
Date of first Publication:2021/05/14
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/04/05
Page Number:14
First Page:4214
Last Page:4227
We are indebted to the European Research Council (ERC) for financial support under the European Union's Horizon 2020 research and innovation program (ACETOGENS, grant agreement no. 741791).
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0