Dual targeting of the tRNA nucleotidyltransferase in plants: not just the signal
- Enzymes involved in tRNA maturation are essential for cytosolic, mitochondrial, and plastid protein synthesis and are therefore localized to these different compartments of the cell. Interestingly, only one isoform of tRNA nucleotidyltransferase (responsible for adding the 3′-terminal cytidine–cytidine–adenosine to tRNAs) has been identified in plants. The present study therefore explored how signals contained on this enzyme allow it to be distributed among the different cell compartments. It is demonstrated that the N-terminal portion of the protein acts as an organellar targeting signal and that differential use of multiple in-frame start codons alters the localization of the protein. Moreover, it is shown that the mature domain has a major impact on the distribution of the protein within the cell. These data indicate that regulation of dual localization involves not only specific N-terminal signals, but also additional factors within the protein or the cell.
Verfasserangaben: | Serena Schmidt von Braun, Antonino Sabetti, Pamela J. Hanic-Joyce, Jun Gu, Enrico SchleiffORCiDGND, Paul B. M. Joyce |
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URN: | urn:nbn:de:hebis:30:3-247819 |
DOI: | https://doi.org/10.1093/jxb/erm267 |
ISSN: | 1460-2431 |
ISSN: | 0022-0957 |
Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/18182422 |
Titel des übergeordneten Werkes (Englisch): | The journal of experimental botany : an official organ of the Society for Experimental Biology |
Verlag: | Oxford Univ. Press |
Verlagsort: | Oxford |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Fertigstellung: | 2012 |
Jahr der Erstveröffentlichung: | 2007 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 11.06.2012 |
Freies Schlagwort / Tag: | Dual targeting; plant protein distribution; tRNA nucleotidyltransferase; targeting signal |
Jahrgang: | 58 |
Ausgabe / Heft: | 15-16 |
Seitenzahl: | 11 |
Erste Seite: | 4083 |
Letzte Seite: | 4093 |
HeBIS-PPN: | 307243435 |
Institute: | Biowissenschaften / Biowissenschaften |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Sammlung Biologie / Sondersammelgebiets-Volltexte | |
Lizenz (Deutsch): | Creative Commons - Namensnennung 3.0 |