Polo-like kinase 1 regulates the stability of the mitotic centromere-associated kinesin in mitosis
- Proper bi-orientation of chromosomes is critical for the accurate segregation of chromosomes in mitosis. A key regulator of this process is MCAK, the mitotic centromere-associated kinesin. During mitosis the activity and localization of MCAK are regulated by mitotic key kinases including Plk1 and Aurora B. We show here that S621 in the MCAK’s C-terminal domain is the major phosphorylation site for Plk1. This phosphorylation regulates MCAK’s stability and facilitates its recognition by the ubiquitin/proteasome dependent APC/CCdc20 pathway leading to its D-box dependent degradation in mitosis. While phosphorylation of S621 does not directly affect its microtubule depolymerising activity, loss of Plk1 phosphorylation on S621 indirectly enhances its depolymerization activity in vivo by stabilizing MCAK, leading to an increased level of protein. Interfering with phosphorylation at S621 causes spindle formation defects and chromosome misalignments. Therefore, this study suggests a new mechanism by which Plk1 regulates MCAK: by regulating its degradation and hence controlling its turnover in mitosis.
Verfasserangaben: | Mourad SanhajiORCiDGND, Andreas Hans RitterORCiDGND, Hannah R. Belsham, Claire T. Friel, Susanne Roth, Frank LouwenORCiDGND, Juping YuanORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-335622 |
DOI: | https://doi.org/10.18632/oncotarget.1861 |
ISSN: | 1949-2553 |
Titel des übergeordneten Werkes (Englisch): | Oncotarget |
Verlag: | Impact Journals LLC |
Verlagsort: | [S.l.] |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Veröffentlichung (online): | 24.03.2014 |
Datum der Erstveröffentlichung: | 24.03.2014 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 30.05.2016 |
Freies Schlagwort / Tag: | MCAK; Plk1; chromosome alignment; protein stability; spindle assembly |
Jahrgang: | 10 |
Ausgabe / Heft: | 5 |
Seitenzahl: | 15 |
Erste Seite: | 3130 |
Letzte Seite: | 3144 |
Bemerkung: | Licensed under a Creative Commons Attribution 3.0 License. http://creativecommons.org/licenses/by/3.0/ |
HeBIS-PPN: | 399892834 |
Institute: | Medizin / Medizin |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Lizenz (Deutsch): | Creative Commons - Namensnennung 3.0 |