Refine
Year of publication
- 2017 (1228) (remove)
Document Type
- Article (1228) (remove)
Language
- English (825)
- German (340)
- Portuguese (40)
- Turkish (8)
- Spanish (7)
- Multiple languages (4)
- French (2)
- Ukrainian (2)
Has Fulltext
- yes (1228)
Is part of the Bibliography
- no (1228)
Keywords
- Deutsch (29)
- Literatur (22)
- Deutsch als Fremdsprache (15)
- Fremdsprachenlernen (15)
- Fremdsprachenunterricht (14)
- Interkulturalität (12)
- Deutschunterricht (11)
- Anarchismus (10)
- Gewalt (10)
- Research article (8)
Institute
- Medizin (330)
- Physik (139)
- Biowissenschaften (68)
- Frankfurt Institute for Advanced Studies (FIAS) (60)
- Gesellschaftswissenschaften (53)
- Biochemie und Chemie (51)
- Präsidium (44)
- Exzellenzcluster Makromolekulare Komplexe (41)
- Institut für Ökologie, Evolution und Diversität (38)
- Institut für Sozialforschung (IFS) (32)
We study the charmonium coherent photoproduction and hadroproduction consistently with modifications from both cold and hot nuclear matters. The strong electromagnetic fields from fast moving nucleus interact with the other target nucleus, producing abundant charmonium in the extremely low transverse momentum region pT<0.1 GeV/c. This results in significative enhancement of J/ψ nuclear modification factor in semi-central and peripheral collisions. In the middle pT region such as pT<3∼5 GeV/c, J/ψ final yield is dominated by the combination process of single charm and anti-charm quarks moving in the deconfined matter, c+c¯→J/ψ+g. In the higher pT region, J/ψ production are mainly from parton initial hard scatterings at the beginning of nucleus–nucleus collisions and decay of B hadrons. We include all of these production mechanisms and explain the experimental data well in different colliding centralities and transverse momentum regions.
Mobilization of hematopoietic stem cells (HSCs) from the bone marrow to the peripheral blood is a complex mechanism that involves adhesive and chemotactic interactions of HSCs as well as their bone marrow microenvironment. In addition to a number of non-genetic factors, genetic susceptibilities also contribute to the mobilization outcome. Identification of genetic factors associated with HSC yield is important to better understand the mechanism behind HSC mobilization. In the present study, we enrolled 148 Korean participants (56 healthy donors and 92 patients) undergoing HSC mobilization for allogeneic or autologous HSC transplantation. Among a total of 53 polymorphisms in 33 candidate genes, one polymorphism (rs11264422) in relaxin/insulin-like family peptide receptor 4 (RXFP4) gene was significantly associated with a higher HSC yield after mobilization in Koreans. However, in a set of 101 Europeans, no association was found between circulating CD34+ cell counts and rs11264422 genotype. Therefore, we suggest that the ethnic differences in subjects’ genetic background may be related to HSC mobilization. In conclusion, the relaxin—relaxin receptor axis may play an important role in HSC mobilization. We believe that the results of the current study could provide new insights for therapies that use relaxin and HSC populations, as well as a better understanding of HSC regulation and mobilization at the molecular level.
No presente artigo analisamos a ideia de solidariedade na teoria da justiça de Axel Honneth. Honneth em sua obra atualiza a teoria hegeliana da eticidade e concebe a existência de esferas de liberdade, sendo que uma delas é a liberdade social, na qual se situam relações pessoais, mercado e instituições políticas. Em O Direito da Liberdade Honneth desenvolve uma teoria em que busca analisar os problemas de desenvolvimento da democracia nas sociedades contemporâneas, e erige a solidariedade como um dos pressupostos normativos para a construção de uma democracia virtuosa, ancorada na participação cidadã.
O presente estudo tem como objetivo investigar a ideia de reconhecimento jurídico na teoria de Axel Honneth, o que se dará mediante análise da obra Luta por Reconhecimento. Honneth, ancorado nas teorias de Hegel e Mead, estabelece o papel do direito como esfera de reconhecimento individual e seu potencial de asseguramento do autorrespeito. Empreende-se uma reconstrução da teoria honnethiana no atinente aos papéis desempenhados pelo direito na teoria da Luta por Reconhecimento de Axel Honneth, para em seguida, a partir da leitura que o autor desenvolve da teoria de Thomas Marshall, analisar o papel desempenhado pelos direitos subjetivos fundamentais como medium de sedimentação e ampliação de novas formas de reconhecimento e cidadania.
Information theory provides a formal framework within which information processing and its disorders can be described. However, information theory has rarely been applied to modeling aspects of the cognitive neuroscience of schizophrenia. The goal of this article is to highlight the benefits of an approach based on information theory, including its recent extensions, for understanding several disrupted neural goal functions as well as related cognitive and symptomatic phenomena in schizophrenia. We begin by demonstrating that foundational concepts from information theory—such as Shannon information, entropy, data compression, block coding, and strategies to increase the signal-to-noise ratio—can be used to provide novel understandings of cognitive impairments in schizophrenia and metrics to evaluate their integrity. We then describe more recent developments in information theory, including the concepts of infomax, coherent infomax, and coding with synergy, to demonstrate how these can be used to develop computational models of schizophrenia-related failures in the tuning of sensory neurons, gain control, perceptual organization, thought organization, selective attention, context processing, predictive coding, and cognitive control. Throughout, we demonstrate how disordered mechanisms may explain both perceptual/cognitive changes and symptom emergence in schizophrenia. Finally, we demonstrate that there is consistency between some information-theoretic concepts and recent discoveries in neurobiology, especially involving the existence of distinct sites for the accumulation of driving input and contextual information prior to their interaction. This convergence can be used to guide future theory, experiment, and treatment development.
Bromus racemosus L. is a rather rare grass species of moist meadows. It has strongly decreased in the course of the 20th century due to intensification of agricultural grassland management, and is therefore included in Red Lists of several European countries. Its winter annual life-cycle is remarkable for a species of permanent grasslands.
The aim of this study is to determine the habitat preference and optimal management of B. racemosus in the Netherlands and surrounding countries. Vegetation, soil and hydrological data from 28 sites in the Netherlands have been compared with B. racemosus cover, and with vegetation data from surrounding countries. The results indicate that B. racemosus is characteristic of Molinio-Arrhenatheretea meadows with good mineralisation and aftermath grazing. The optimum lies in grasslands of the alliance Alopecurion pratensis (Deschampsion cespitosae), but the species ranges from wetter Calthion palustris meadows to drier Arrhenatherion elatioris and Cynosurion cristati grasslands. It prefers intermediate nutrient levels and hydrological conditions (mesic sites), but within this range the highest cover is found in relatively nutrient rich and dry sites. Because of the absence of a seedbank and a low dispersal capability, B. racemosus is vulnerable to changes in grassland management. A management of mowing after 15 June and aftermath grazing is most suitable, since it enables fruit ripening and the maintenance of an open sward, needed for germination and development. The risk of extinction is likely to be higher in flat polders than in floodplain sites with natural relief, where the species may shift between belts in different years.
Im Jahr 1926 erschien in der 'Revista de Occidente', einer von José Ortega y Gasset in Madrid herausgegebenen Zeitschrift mit kulturpolitischer und philosophischer Ausrichtung, eine merkwürdige Publikation unter dem rätselhaften Titel "Cancionero apócrifo de Abel Martín. Recopilación y estudio de Juan de Mairena." Auf den ersten Blick scheint es so, als fungiere Antonio Machado hier lediglich als Herausgeber einer von Juan de Mairena kompilierten Sammlung, welche wiederum der Autorschaft eines Abel Martín zugeschrieben wird. Trotz der rätselhaften doppelten Herausgeberfiktion hatte das damalige Lesepublikum offenbar keine Probleme damit, Antonio Machado als den eigentlichen Autor der genannten "apokryphen" Prosa- und Gedichttexte zu identifizieren. Denn die Namen Juan de Mairena und Abel Martín bezeichnen nicht etwa reale Dichterpersönlichkeiten der damaligen Epoche oder der spanischen Literatur- geschichte, sondern sie sind – wie die damaligen Leser durch Ausschlussverfahren selbst erraten konnten – Konstrukte von Machados Phantasie, fiktive Autorprojektionen, deren Erfindung und Gestaltung er mit erstaunlichem gedanklichen Aufwand und Akribie betrieben hatte. Abel Martín und sein 'Schüler' Mairena gehören zu einem ganzen Spektrum von imaginierten Dichtern und Philosophen, deren fingierte Werke ihr Erfinder Machado als "apokryph" bezeichnete. Die bemerkenswerte Praktik, eigene Hervorbringungen, Lyrik und Prosa, nicht unter dem eigenen Namen zu publizieren, sondern einem Kreis fingierter Autorpersönlichkeiten zuzuordnen, hebt sich von dem gewöhnlichen Gebrauch von Pseudonymen, von künstlerischen Decknamen, deutlich ab. Zumal der Autor in jenem Zeitraum in den 1910er und 1920er Jahren keineswegs mehr ein Unbekannter war.
Dem Apokryphen einen Themenschwerpunkt im Rahmen einer komparatistischen Zeitschrift zu widmen ist eine Entscheidung, die auf den ersten Blick erstaunen mag. Ist doch das Wort "apokryph" ein in der heutigen Alltagssprache wenig geläufiger Begriff, der, auf das Register der Theologie verweisend, speziellen Fragen und fachwissenschaftlichen Debatten der Religionsgeschichte vorbehalten zu sein scheint. Wenn wir gleichwohl Begriff und Phänomen des Apokryphen als Schwerpunktthema des Jahrbuchs 'Komparatistik' zur Diskussion stellen wollen, so deshalb, weil der damit angesprochene Sachverhalt von Prozessen der Auswahl, Marginalisierung und Ausgrenzung im Rahmen kultureller und textueller Überlieferung ein Problemfeld markiert, das im Bereich der Literaturgeschichte vielfältige Resonanzen und Parallelen findet und in systematischer Hinsicht über Verfahren und Mechanismen literarischer Traditionsbildung Aufschluss zu geben verspricht. Weit davon entfernt, nur ein spezialdiskursives Sonderphänomen zu sein, erweist sich das Apokryphe bei näherem Hinsehen als eine Figur, an der sich grundsätzliche Fragen der Formation von Text- und Wissensbeständen, der literarischen Autorität und Kanonbildung beobachten lassen. Apokryphe Texte, so ließe sich in einer vorläufigen, im Folgenden noch zu präzisierenden Beschreibung formulieren, sind Texte, die sich am Rande der großen Traditionen religiöser und kultureller Bewegungen situieren. Es sind Texte oder Textensembles, die es, wie sich im Rückblick bemerken lässt, nicht geschafft haben, in das Inventar jener Schriften aufgenommen zu werden, die als anerkannt, bewahrenswert oder wahr gelten. Als Einstieg bietet es sich an, zunächst von den Bedeutungsdimensionen des Ausdrucks apokryph auszugehen, die dieser im Kontext der religionsgeschichtlichen Tradition angenommen hat und der für das heute übliche Verständnis des Begriffs bestimmend ist. Der religiöse bzw. theologische Begriffsgebrauch greift auf das altgriechische Wort ἀπόκρυφος (apókryphos) zurück, um dieses vor allem in einer seiner beiden Bedeutungsschichten zum Einsatz zu bringen, nämlich als Bezeichnung eines Gegenstands bzw. Textes, der als 'unecht', 'zweifelhaft', 'nicht-authentisch' zu gelten habe. Der Begriff apokryph führt hier also eine Unterscheidung ein zwischen dem, was als gesichert, gültig und autorisiert anzusehen ist und dem, was solche Geltung zu besitzen nicht oder nur unter dem Vorbehalt des Zweifelhaften beanspruchen kann. Bei dem Begriffspaar gültig vs. apokryph haben wir es also mit einer Figur der Verknappung zu tun, die ein gegebenes Textensemble oder eine kulturelle Überlieferung einem mitunter radikalen Verfahren der Auswahl und Reduktion unterzieht.
The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build in the mitochondrial inner membrane various supramolecular assemblies. The formation of the individual complexes and their supercomplexes depends on the activity of dedicated assembly factors. We identified a so far uncharacterized mitochondrial protein (open reading frame YDR381C-A) as an important assembly factor for complex III, complex IV, and their supercomplexes. Therefore, we named this protein Cox interacting (Coi) 1. Deletion of COI1 results in decreased respiratory growth, reduced membrane potential, and hampered respiration, as well as slow fermentative growth at low temperature. In addition, coi1Δ cells harbour reduced steady-state levels of subunits of complexes III and IV as well as of the assembled complexes and supercomplexes. Interaction of Coi1 with respiratory chain subunits seems transient, as it appears to be a stoichiometric subunit neither of complex III nor of complex IV. Collectively, this work identifies a novel protein that plays a role in the assembly of the mitochondrial respiratory chain.
Most event studies rely on cumulative abnormal returns, measured as percentage changes in stock prices, as their dependent variable. Stock price reflects the value of the operating business plus non-operating assets minus debt. Yet, many events, in particular in marketing, only influence the value of the operating business, but not non-operating assets and debt. For these cases, the authors argue that the cumulative abnormal return on the operating business, defined as the ratio between the cumulative abnormal return on stock price and the firm-specific leverage effect, is a more appropriate dependent variable. Ignoring the differences in firm-specific leverage effects inflates the impact of observations pertaining to firms with large debt and deflates those pertaining to firms with large non-operating assets. Observations of firms with high debt receive several times the weight attributed to firms with low debt. A simulation study and the reanalysis of three previously published marketing event studies shows that ignoring the firm-specific leverage effects influences an event study's results in unpredictable ways.
The physical housing environment is important to facilitate activities of daily living (ADL) for older people. A hindering environment may lead to ADL dependence and thus increase the need for home services, which is individually restricting and a growing societal burden. This study presents simulations of policy changes with regard to housing accessibility that estimates the potential impact specifically on instrumental activities of daily living (I-ADL), usage of home services, and related costs. The models integrate empirical data to test the hypothesis that a policy providing funding to remove the five most severe environmental barriers in the homes of older people who are at risk of developing dependence in I-ADL, can maintain independence and reduce the need for home services. In addition to official statistics from state agencies in Sweden and Germany, we utilized published results from the ENABLE-AGE and other scientific studies to generate the simulations. The simulations predicted that new policies that remove potentially hindering housing features would improve I-ADL performance among older people and reduce the need for home services. Our findings suggest that a policy change can contribute to positive effects with regard to I-ADL independence among older people and to a reduction of societal burden.
Vegetation patterns of floodplain forests are highly variable across different habitats in European regions. Their plant communities have been well described from the phytosociological point of view, but plant species richness and composition patterns and their underlying environmental factors are still insufficiently known. Sixty-one vegetation plots of black alder-dominated floodplain forests were sampled in central Slovakia in order to find main environmental predictors affecting floristic diversity of their vegetation. For each vegetation plot with a constant size (400m2), vascular plant species and a set of topographic, climatic, soil physical and chemical characteristics were recorded. A generalized linear model was applied to explain relevance of environmental factors on changes of species richness, whereas the relationship between species composition and explanatory variables was tested using ordination methods. Main gradients of species compositional variation were soil moisture, light, elevation and soil chemistry-related variables. Vascular species richness of plots varied between 19 and 59 (mean 38). Herb-layer species richness was positively related to the soil pH, stream power index and negatively to the concentration of soil iron. These linear trends were accompanied by a hump-shaped response to sand content and a U-shaped response to elevation.
Sex differences in the relationship between conduct disorder and cortical structure in adolescents
(2017)
Objective: Previous studies have reported reduced cortical thickness and surface area and altered gyrification in frontal and temporal regions in adolescents with conduct disorder (CD). Although there is evidence that the clinical phenotype of CD differs between males and females, no studies have examined whether such sex differences extend to cortical and subcortical structure.
Method: As part of a European multisite study (FemNAT-CD), structural magnetic resonance imaging (MRI) data were collected from 48 female and 48 male participants with CD and from 104 sex-, age-, and pubertal-status−matched controls (14–18 years of age). Data were analyzed using surface-based morphometry, testing for effects of sex, diagnosis, and sex-by-diagnosis interactions, while controlling for age, IQ, scan site, and total gray matter volume.
Results: CD was associated with cortical thinning and higher gyrification in ventromedial prefrontal cortex in both sexes. Males with CD showed lower, and females with CD showed higher, supramarginal gyrus cortical thickness compared with controls. Relative to controls, males with CD showed higher gyrification and surface area in superior frontal gyrus, whereas the opposite pattern was seen in females. There were no effects of diagnosis or sex-by-diagnosis interactions on subcortical volumes. Results are discussed with regard to attention-deficit/hyperactivity disorder, depression, and substance abuse comorbidity, medication use, handedness, and CD age of onset.
Conclusion: We found both similarities and differences between males and females in CD–cortical structure associations. This initial evidence that the pathophysiological basis of CD may be partly sex-specific highlights the need to consider sex in future neuroimaging studies and suggests that males and females may require different treatments.
The existence of individual variation in males' motivation to mate remains a conundrum as directional selection should favour high mating frequencies. Balancing selection resulting from (context-dependent) female mate choice could contribute to the maintenance of this behavioural polymorphism. In dichotomous choice tests, mosquitofish (Gambusia holbrooki) females preferred virtual males showing intermediate mating frequencies, reflecting females' tendencies to avoid harassment by highly sexually active males. When tested in the presence of a female shoal—which protects females from male harassment—focal females showed significantly stronger preferences for high sexual activity. A trade-off between (indirect) benefits and (direct) costs of mating with sexually active males probably explains context-dependent female mate choice, as costs depend on the social environment in which females choose their mates. No preference was observed when we tested virgin females, suggesting that the behavioural pattern described here is part of the learned behavioural repertoire of G. holbrooki females.
BACKGROUND: Time-limited, early-life exposures to institutional deprivation are associated with disorders in childhood, but it is unknown whether effects persist into adulthood. We used data from the English and Romanian Adoptees study to assess whether deprivation-associated adverse neurodevelopmental and mental health outcomes persist into young adulthood.
METHODS: The English and Romanian Adoptees study is a longitudinal, natural experiment investigation into the long-term outcomes of individuals who spent from soon after birth to up to 43 months in severe deprivation in Romanian institutions before being adopted into the UK. We used developmentally appropriate standard questionnaires, interviews completed by parents and adoptees, and direct measures of IQ to measure symptoms of autism spectrum disorder, inattention and overactivity, disinhibited social engagement, conduct or emotional problems, and cognitive impairment (IQ score <80) during childhood (ages 6, 11, and 15 years) and in young adulthood (22-25 years). For analysis, Romanian adoptees were split into those who spent less than 6 months in an institution and those who spent more than 6 months in an institution. We used a comparison group of UK adoptees who did not experience deprivation. We used mixed-effects regression models for ordered-categorical outcome variables to compare symptom levels and trends between groups.
FINDINGS: Romanian adoptees who experienced less than 6 months in an institution (n=67 at ages 6 years; n=50 at young adulthood) and UK controls (n=52 at age 6 years; n=39 at young adulthood) had similarly low levels of symptoms across most ages and outcomes. By contrast, Romanian adoptees exposed to more than 6 months in an institution (n=98 at ages 6 years; n=72 at young adulthood) had persistently higher rates than UK controls of symptoms of autism spectrum disorder, disinhibited social engagement, and inattention and overactivity through to young adulthood (pooled p<0·0001 for all). Cognitive impairment in the group who spent more than 6 months in an institution remitted from markedly higher rates at ages 6 years (p=0·0001) and 11 years (p=0·0016) compared with UK controls, to normal rates at young adulthood (p=0·76). By contrast, self-rated emotional symptoms showed a late-onset pattern with minimal differences versus UK controls at ages 11 years (p=0·0449) and 15 years (p=0·17), and then marked increases by young adulthood (p=0·0005), with similar effects seen for parent ratings. The high deprivation group also had a higher proportion of people with low educational achievement (p=0·0195), unemployment (p=0·0124), and mental health service use (p=0·0120, p=0·0032, and p=0·0003 for use when aged <11 years, 11-14 years, and 15-23 years, respectively) than the UK control group. A fifth (n=15) of individuals who spent more than 6 months in an institution were problem-free at all assessments.
INTERPRETATION: Notwithstanding the resilience shown by some adoptees and the adult remission of cognitive impairment, extended early deprivation was associated with long-term deleterious effects on wellbeing that seem insusceptible to years of nurturance and support in adoptive families.
FUNDING: Economic and Social Research Council, Medical Research Council, Department of Health, Jacobs Foundation, Nuffield Foundation.
Este artigo apresenta uma discussão sobre a obra recente de Axel Honneth. Começando por uma exposição de seu projeto teórico mais amplo, descrito em O direito da liberdade (2011) como uma teoria da justiça sociologicamente ancorada, o artigo demonstra como Honneth se move da ideia de uma luta por reconhecimento em direção à ideia de intersubjetividade institucional. Este movimento, porém, é acompanhado por críticas que vêm nesta passagem o abandono das ambições críticas de seu modelo e um compromisso reformista com a ordem capitalista de mercado. A fim de responder a estas objeções, Honneth propõe uma reatualização da ideia do socialismo (2015) a partir da noção de liberdade social, concluindo com uma distinção entre duas formas de luta por reconhecimento, uma interna e outra externa – e defendendo as vantagens da primeira.
From reciprocal recognition to a society that is properly 'social' : on Axel Honneth's recent work
(2017)
This paper addresses Axel Honneth's recent endeavors to defend his theory of justice, broadly described in Freedom's right (2011) as an analysis of society. The paper begins by exposing Honneth's model as a theory of institutional intersubjectivity rather than a theory of the struggle for recognition. This model, however, was subject to criticism due to its supposed acceptance of the capitalistic market economy as a social order. In order to defend it from such objections, Honneth (2016) exposes the normative core of socialist ideals as a version of social freedom. Finally, he presents a distinction between two forms of political intervention: an internal and an external struggle for recognition – and asserts the advantages of the former.
Auf Grundlage einer interviewbasierten Studie zu heterosexuellen Paaren, in denen die Frau das Haupteinkommen verdient, beschäftigt sich der Beitrag mit milieuspezifischen Bewältigungsmustern prekärer Beschäftigungsverhältnisse. Vor dem Hintergrund der Erosion des Ernährermodells werden dabei Transformationen von Männlichkeit in den Blick genommen. Es wird die These entwickelt, dass sich mit dem Selbstverständnis als "Künstler" im hochqualifizierten individualisierten Milieu des urbanen Raums ein spezifisches Bewältigungsmuster von Prekarität herausgebildet hat.
Correlation of lumbar lateral recess stenosis in magnetic resonance imaging and clinical symptoms
(2017)
Aim: To assess the correlation of lateral recess stenosis (LRS) of lumbar segments L4/5 and L5/S1 and the Oswestry Disability Index (ODI).
Methods: Nine hundred and twenty-seven patients with history of low back pain were included in this uncontrolled study. On magnetic resonance images (MRI) the lateral recesses (LR) at lumbar levels L4/5 and L5/S1 were evaluated and each nerve root was classified into a 4-point grading scale (Grade 0-3) as normal, not deviated, deviated or compressed. Patient symptoms and disability were assessed using ODI. The Spearman’s rank correlation coefficient was used for statistical analysis (P < 0.05).
Results: Approximately half of the LR revealed stenosis (grade 1-3; 52% at level L4/5 and 42% at level L5/S1) with 2.2% and 1.9% respectively reveal a nerve root compression. The ODI score ranged from 0%-91.11% with an arithmetic mean of 34.06% ± 16.89%. We observed a very weak statistically significant positive correlation between ODI and LRS at lumbar levels L4/5 and L5/S1, each bilaterally (L4/5 left: rho < 0.105, P < 0.01; L4/5 right: rho < 0.111, P < 0.01; L5/S1 left: rho 0.128, P < 0.01; L5/S1 right: rho < 0.157, P < 0.001).
Conclusion: Although MRI is the standard imaging tool for diagnosing lumbar spinal stenosis, this study showed only a weak correlation of LRS on MRI and clinical findings. This can be attributed to a number of reasons outlined in this study, underlining that imaging findings alone are not sufficient to establish a reliable diagnosis for patients with LRS.
In Europe, anthroponyms have undergone a complex development, as simple one-word personal names have developed into multi-word structures containing one, two or more personal names and a stable surname. The article predominantly characterizes the development of surnames on the basis of their origin. Attention is also paid to different trends in the development of personal names. The article analyses this phenomenon from various perspectives, using an example of one specific living person’s anthroponyms.
Nerve tissue contains a high density of chemical synapses, about 1 per µm3 in the mammalian cerebral cortex. Thus, even for small blocks of nerve tissue, dense connectomic mapping requires the identification of millions to billions of synapses. While the focus of connectomic data analysis has been on neurite reconstruction, synapse detection becomes limiting when datasets grow in size and dense mapping is required. Here, we report SynEM, a method for automated detection of synapses from conventionally en-bloc stained 3D electron microscopy image stacks. The approach is based on a segmentation of the image data and focuses on classifying borders between neuronal processes as synaptic or non-synaptic. SynEM yields 97% precision and recall in binary cortical connectomes with no user interaction. It scales to large volumes of cortical neuropil, plausibly even whole-brain datasets. SynEM removes the burden of manual synapse annotation for large densely mapped connectomes.
Background: Infections with the hepatitis C virus (HCV) are a global public health problem. Long-term consequences are the development of liver cirrhosis and hepatocellular carcinoma. Newly introduced direct acting antivirals, especially interferon-free regimens, have improved rates of sustained viral response above 90% in most patient groups and allow treating patients who were ineligible for treatment in the past. These new regimens have replaced former treatment and are recommended by current guidelines. However, there is an ongoing discussion on high pharmaceutical prices. Our aim was to assess the long-term cost-effectiveness of treating hepatitis C genotype 1 patients with sofosbuvir/ledipasvir (SOF/LDV) treatment in Germany.
Material and Methods: We used a Markov cohort model to simulate disease progression and assess cost-effectiveness. The model calculates lifetime costs and outcomes (quality-adjusted life years, QALYs) of SOF/LDV and other strategies. Patients were stratified by treatment status (treatment-naive and treatment-experienced) and absence/presence of cirrhosis. Different treatment strategies were compared to prior standard of care. Sensitivity analyses were performed to evaluate model robustness.
Results: Base-case analyses results show that in treatment-naive non-cirrhotic patients treatment with SOF/LDV dominates the prior standard of care (is more effective and less costly). In cirrhotic patients an incremental cost-effectiveness ratio (ICER) of 3,383 €/QALY was estimated. In treatment-experienced patients ICERs were 26,426 €/QALY and 1,397 €/QALY for treatment-naive and treatment-experienced patients, respectively. Robustness of results was confirmed in sensitivity analyses.
Conclusions: Our analysis shows that treatment with SOF/LDV is cost-effective compared to prior standard of care in all patient groups considering international costs per QALY thresholds.
The accurate knowledge of the neutron-induced fission cross-sections of actinides and other isotopes involved in the nuclear fuel cycle is essential for the design of advanced nuclear systems, such as Generation-IV nuclear reactors. Such experimental data can also provide the necessary feedback for the adjustment of nuclear model parameters used in the evaluation process, resulting in the further development of nuclear fission models. In the present work, the 240Pu(n,f) cross-section was measured at CERN's n_TOF facility relative to the well-known 235U(n,f) cross section, over a wide range of neutron energies, from meV to almost MeV, using the time-of-flight technique and a set-up based on Micromegas detectors. This measurement was the first experiment to be performed at n_TOF's new experimental area (EAR-2), which offers a significantly higher neutron flux compared to the already existing experimental area (EAR-1). Preliminary results as well as the experimental procedure, including a description of the facility and the data handling and analysis, are presented.
Tumor cells frequently overexpress heat shock protein 70 (Hsp70) and present it on their cell surface, where it can be recognized by pre‐activated NK cells. In our retrospective study the expression of Hsp70 was determined in relation to tumor‐infiltrating CD56+ NK cells in formalin‐fixed paraffin embedded (FFPE) tumor specimens of patients with SCCHN (N = 145) as potential indicators for survival and disease recurrence. All patients received radical surgery and postoperative cisplatin‐based radiochemotherapy (RCT). In general, Hsp70 expression was stronger, but with variable intensities, in tumor compared to normal tissues. Patients with high Hsp70 expressing tumors (scores 3–4) showed significantly decreased overall survival (OS; p = 0.008), local progression‐free survival (LPFS; p = 0.034) and distant metastases‐free survival (DMFS; p = 0.044), compared to those with low Hsp70 expression (scores 0–2), which remained significant after adjustment for relevant prognostic variables. The adverse prognostic value of a high Hsp70 expression for OS was also observed in patient cohorts with p16‐ (p = 0.001), p53‐ (p = 0.0003) and HPV16 DNA‐negative (p = 0.001) tumors. The absence or low numbers of tumor‐infiltrating CD56+ NK cells also correlated with significantly decreased OS (p = 0.0001), LPFS (p = 0.0009) and DMFS (p = 0.0001). A high Hsp70 expression and low numbers of tumor‐infiltrating NK cells have the highest negative predictive value (p = 0.00004). In summary, a strong Hsp70 expression and low numbers of tumor‐infiltrating NK cells correlate with unfavorable outcome following surgery and RCT in patients with SCCHN, and thus serve as negative prognostic markers.
We are pleased to introduce the sixth Journal Club. This edition is focused on several relevant studies published in the last years in the field of Strength and Conditioning, chosen by our Editorial Board members and their colleagues. We hope to stimulate your curiosity in this field and to share with you the passion for sport and exercise seen from a scientific point of view. The Editorial Board members wish you an inspiring read.
Laut jüdischem Kalender entstand die Welt vor genau 5778 Jahren, nach der Bibel vor 6021 Jahren. Doch als Forscher begannen, auf und in der Erde selbst nach Spuren ihres Alters zu suchen, mussten sie die Zahl immer weiter nach oben korrigieren. Nach heutigen Datierungsmethoden ist unser Planet zwischen 4,5 und 4,6 Milliarden Jahre alt.
Auf der Gamescom waren dieses Jahr neben Besuchern und Spieleentwicklern auch die politischen Parteien vertreten. In der "Wahlkampfarena" ging es um den Status von Videospielen, Breitbandausbau und digitale Bildung. Die Debatte im Überblick.
... Anwesend waren die Generalsekretäre bzw. Bundesgeschäftsführer der Parteien des aktuellen Bundestages, Peter Tauber (CDU), Hubertus Heil (SPD), Matthias Höhn (Die Linke) und Michael Kellner (Die Grünen) sowie Nicola Beer für die FDP. ...
In bacteria, the regulation of gene expression by cis-acting transcriptional riboswitches located in the 5'-untranslated regions of messenger RNA requires the temporal synchronization of RNA synthesis and ligand binding-dependent conformational refolding. Ligand binding to the aptamer domain of the riboswitch induces premature termination of the mRNA synthesis of ligand-associated genes due to the coupled formation of 3'-structural elements acting as terminators. To date, there has been no high resolution structural description of the concerted process of synthesis and ligand-induced restructuring of the regulatory RNA element. Here, we show that for the guanine-sensing xpt-pbuX riboswitch from Bacillus subtilis, the conformation of the full-length transcripts is static: it exclusively populates the functional off-state but cannot switch to the on-state, regardless of the presence or absence of ligand. We show that only the combined matching of transcription rates and ligand binding enables transcription intermediates to undergo ligand-dependent conformational refolding.
Die Verkleidung des Richters und die Verkleidung der Muslima sind ähnlich, weil sie beide aus einer Abschirmung rühren, die auf gleiche Weise dogmatisch besetzt ist. Auch wenn, zumindest in der deutschen Übersetzung des Koran, mit der Bedeckung der Haare die Keuschheit, Sittsamkeit oder Schamhaftigkeit der Frau und mit der Verkleidung des Richters seine Neutralität symbolisiert oder umgesetzt werden soll, gibt es doch eine Entsprechung, und die liegt in der Abschirmung. Wenn man hijab mit Absperrung oder Verhüllung übersetzt, wie das einige vorschlagen, dann tragen Richter auch einen hijab.
Die gleichgeschlechtliche Ehe wurde vom Gesetzgeber endlich anerkannt, auch das Verfassungsgericht wird dagegen nichts mehr unternehmen können, das hoffe ich zumindest. Wer seine Zeichen und Gesetze dennoch exklusiv halten will, sollte darum darüber nachdenken, auf Alternativbegriffe zur Ehe umzusteigen.
Wozu VB?
(2017)
Die eifrigste Bestrebung der Kinder besteht darin, so hat es zumindest Sigmund Freud einmal behauptet, "zu erfahren, was die Eltern miteinander tun, woraus dann die Kinder werden." Was treibt die Kinder in die Welt? Haben die Kinder Juristen als Eltern, dann dürften sie bald eher nach den Gründen als nach dem Treiben fragen.
Wenn Rechtstexte auf literarische Texte treffen, dann treffen Wahrheitsformen aufeinander. Es treffen unterschiedliche Weisen, Wahrheit zu produzieren, aufeinander. Zu den zahlreichen Unterschieden gehören, historisch bedingt, unterschiedliche Stile, die sich um die Objektivierung und "Subjektivierung" der Aussagen bilden. Das fängt bei den banalen "wir" und "man" rechtswissenschaftlicher Texte an, geht über allgemein gehaltene, enthistorisierende und systematisierende Definitionen bis zu einem Fussnotenapparat, der in manchen Rechtstexten beinahe jede Aussage als nachweisbare Aussage absichern soll. Die Rechtswissenschaft, zumal die deutsche, pflegt bei ihren Wahrheitsformen objektivierende Stile, die Literaturwissenschaft tut das nicht, nicht in dem Maße, sie lässt das Subjekt stärker in die Aussage einbrechen. Die Lage zwischen diesen beiden Disziplinen ist allerdings kompliziert, weil wiederum das Recht eine wirksame Subjektivierungsinstanz ist, die eben die Instrumente zur Verfügung stellt, um Aussagen an Subjekte zu binden. Sie hat die Unterschrift und den Urheber erfunden. In dieser Lage kann man also nicht einfach das Subjektive gegen das Objektive ausspielen. Von Anfang aber über das Zusammentreffen von Literatur und Recht in objektivierenden Stilen zu schreiben, schafft eine asymmetrische Ausgangslage, die ich gerne zugunsten einer symmetrischen Ausgangslage umgehen möchte – soweit das möglich ist. ...
We study the correlation between the distributions of the net-charge, net-kaon, net-baryon and net-proton number at hadronization and after the final hadronic decoupling by simulating ultra relativistic heavy ion collisions with the hybrid version of the ultrarelativistic quantum molecular dynamics (UrQMD) model. We find that due to the hadronic rescattering these distributions are not strongly correlated. The calculated change of the correlation, during the hadronic expansion stage, does not support the recent paradigm, namely that the measured final moments of the experimentally observed distributions do give directly the values of those distributions at earlier times, when the system had been closer to the QCD crossover.
Chromosomal translocations - leading to the expression of fusion genes - are well-studied genetic abberrations associated with the development of leukemias. Most of them represent altered transcription factors that affect transcription or epigenetics, while others - like BCR-ABL - are enhancing signaling. BCR-ABL has become the prototype for rational drug design, and drugs like Imatinib and subsequently improved drugs have a great impact on cancer treatments. By contrast, MLL-translocations in acute leukemia patients are hard to treat, display a high relapse rate and the overall survival rate is still very poor. Therefore, new treatment modalities are urgently needed. Based on the molecular insights of the most frequent MLL rearrangements, BET-, DOT1L-, SET- and MEN1/LEDGF-inhibitors have been developed and first clinical studies were initiated. Not all results of these studies have are yet available, however, a first paper reports a failure in the DOT1L-inhibitor study although it was the most promising drug based on literature data. One possible explanation is that all of the above mentioned drugs also target the cognate wildtype proteins. Here, we want to strengthen the fact that efforts should be made to develop drugs or strategies to selectively inhibit only the fusion proteins. Some examples will be given that follow exactly this guideline, and proof-of-concept experiments have already demonstrated their feasibility and effectiveness. Some of the mentioned approaches were using drugs that are already on the market, indicating that there are existing opportunities for the future which should be implemented in future therapy strategies.
Nach den Ereignissen in Gaggenau liest und hört man allenthalben von den "rechtlichen Schwierigkeiten", die hinsichtlich Verboten von Redeauftritten ausländischer Politiker bestünden. In Gaggenau hat man originär sicherheitsrechtlich argumentiert: viel zu viele Leute, viel zu kleiner Parkplatz, Chaos vorprogrammiert. Ähnliches nun in Köln: zu großer Aufwand, zu kurzfristig, Chaos vorprogrammiert. Nehmen wir einmal an, es seien im Einzelfall tragfähige Begründungen gewesen. Dann stellt sich zugleich die Frage: Was kann man tun, wenn man den Auftritt eines ausländischen Vertreters untersagen will, obwohl der Parkplatz groß genug und die Polizei ausreichend gegen Ausschreitungen gewappnet ist? ...
Nachruf auf John Neubauer
(2017)
Am 5. Oktober 2015 ist in Amsterdam der Germanist und Komparatist John Neubauer verstorben, an einer erst kurz zuvor diagnostizierten und tapfer ertragenen schweren Krankheit, im Kreise seiner Angehörigen und im Alter von nicht ganz zweiundachtzig Jahren. Unser Fach verliert in ihm einen hervorragenden Vertreter, und alle, die ihn kannten, verlieren einen hochsympathischen und inspirierenden Mitmenschen.
Der vorliegende Beitrag beschäftigt sich mit einem Gedicht Heiner Müllers, das dieser in unmittelbarer Reaktion auf den Zusammenbruch des osteuropäischen Sozialismus verfasste. Da dieses Gedicht in mehreren Etappen geschrieben und publiziert wurde, gibt es Einblicke in die von Umbruch und Verwerfungen gekennzeichnete Entstehungssituation, die der Beitrag erläutern will.
Es kann gleichermaßen als eine vorläufige Lebensbilanz des Autors gelesen werden, wo sich selbstkritische Äußerungen zu den Irrtümern mit denen einer geschichtlichen Epoche kreuzen. Da die eingenommene Haltung zu diesem komplexen Veränderungsprozess und die Bewertung dieser Wende durch den Autor auch wiederum einer Selbstkritik unterworfen wurde, versucht der Beitrag, einen Einblick in die geschichtsphilosophische und ästhetische Werkstatt des Autors Heiner Müller zu geben
Treatment of refractory ascites with an automated low-flow ascites pump in patients with cirrhosis
(2017)
Background: Refractory ascites (RA) is a frequent complication of cirrhosis, requiring large volume paracentesis or placement of a transjugular intrahepatic portosystemic shunt (TIPSS). The automated low-flow ascites pump (alfapump, Sequana Medical AG, Zurich, Switzerland) is an innovative treatment option for patients with RA.
Aim: To assess safety and efficacy of this treatment in patients with a contraindication to TIPSS.
Methods: Fifty-six patients (43 males; mean age 62 years) from centres in Germany, Switzerland, UK and Spain were included and followed for up to 24 months. Complications, device deficiencies, paracentesis frequency and patient survival were recorded.
Results: At the time of this analysis, 3 patients completed the 24-month observation period, monitoring of 3 was ongoing, 9 underwent liver transplantation, 17 patients were withdrawn due to serious adverse events and 23 patients died. Most frequently observed technical complication was blocking of the peritoneal catheter. Twenty-three pump-related reinterventions (17 patients) and 12 pump exchanges (11 patients) were required during follow-up. The pump system was explanted in 48% of patients (in 17 patients due to serious adverse events, in 9 at the time of liver transplantation and in 1 due to recovery from RA). Median frequency of paracentesis dropped from 2.17 to 0.17 per month.
Conclusions: The alfapump can expand therapeutic options for cirrhotic patients with RA. Continuous drainage of ascites in a closed loop automated system led to significant reduction in paracentesis frequency. Technical and procedural improvements are required to reduce the rate of adverse events and reinterventions.
Das Recht – abstrakt und bilderfeindlich? Ein Fehlurteil. Denn schon immer hat das Recht zu sinnlichen Hilfsmitteln gegriffen, um sich den Menschen verständlich zu machen, teils auf realer, besonders gern aber auf sprachlicher Ebene. Die heutige Bilderflut ist jedoch auch für die Rechtswissenschaft ein neues Phänomen.
Anticoagulation with warfarin and rivaroxaban ameliorates experimental autoimmune encephalomyelitis
(2017)
Background: In multiple sclerosis, coagulation factors have been shown to modulate inflammation. In this translational study, we investigated whether long-term anticoagulation with warfarin or rivaroxaban has beneficial effects on the course of autoimmune experimental encephalomyelitis (EAE).
Methods: Female SJL/J mice treated with anticoagulants namely warfarin or rivaroxaban were immunized with PLP139–151. Stable anticoagulation was maintained throughout the entire experiment. Mice without anticoagulation treated with the vehicle only were used as controls. The neurological deficit was recorded during the course of EAE, and histopathological analyses of inflammatory lesions were performed.
Results: In preventive settings, both treatment with warfarin and rivaroxaban reduced the maximum EAE score as compared to the control group and led to a reduction of inflammatory lesions in the spinal cord. In contrast, therapeutic treatment with warfarin had no beneficial effects on the clinical course of EAE. Signs of intraparenchymal hemorrhage at the site of the inflammatory lesions were not observed.
Conclusion: We developed long-term anticoagulation models that allowed exploring the course of EAE under warfarin and rivaroxaban treatment. We found a mild preventive effect of both warfarin and rivaroxaban on neurological deficits and local inflammation, indicating a modulation of the disease induction by anticoagulation.
Nutrition support is a necessary therapy for critically ill cardiac surgery patients. However, conclusive evidence for this population, consisting of well-conducted clinical trials is lacking. To clarify optimal strategies to improve outcomes, an international multidisciplinary group of 25 experts from different clinical specialties from Germany, Canada, Greece, USA and Russia discussed potential approaches to identify patients who may benefit from nutrition support, when best to initiate nutrition support, and the potential use of pharmaco-nutrition to modulate the inflammatory response to cardiopulmonary bypass. Despite conspicuous knowledge and evidence gaps, a rational nutritional support therapy is presented to benefit patients undergoing cardiac surgery.
Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays to enable directional gene trap cassette inversions by Cre and Flpe site-specific recombinases, greatly enhanced their experimental potential. By taking advantage of these conditional gene trap cassettes, we developed a generic strategy for generating conditional mutations and validated this strategy in mice carrying a multipurpose allele of the Prdm16 transcription factor gene. We demonstrate that the gene trap insertion creates a null mutation replicating the Pierre Robin sequence-type cleft palate phenotype of other Prdm16 mutant mice. Consecutive breeding to Flpe and Emx1IREScre deleter mice spatially restricted Prdm16 loss to regions of the forebrain expressing the homeobox gene Emx1, demonstrating the utility of the technology for the analysis of tissue-specific gene functions.
Background: In oldest-old patients (>80), few trials showed efficacy of treating hypertension and they included mostly the healthiest elderly. The resulting lack of knowledge has led to inconsistent guidelines, mainly based on systolic blood pressure (SBP), cardiovascular disease (CVD) but not on frailty despite the high prevalence in oldest-old. This may lead to variation how General Practitioners (GPs) treat hypertension. Our aim was to investigate treatment variation of GPs in oldest-olds across countries and to identify the role of frailty in that decision.
Methods: Using a survey, we compared treatment decisions in cases of oldest-old varying in SBP, CVD, and frailty. GPs were asked if they would start antihypertensive treatment in each case. In 2016, we invited GPs in Europe, Brazil, Israel, and New Zealand. We compared the percentage of cases that would be treated per countries. A logistic mixed-effects model was used to derive odds ratio (OR) for frailty with 95% confidence intervals (CI), adjusted for SBP, CVD, and GP characteristics (sex, location and prevalence of oldest-old per GP office, and years of experience). The mixed-effects model was used to account for the multiple assessments per GP.
Results: The 29 countries yielded 2543 participating GPs: 52% were female, 51% located in a city, 71% reported a high prevalence of oldest-old in their offices, 38% and had >20 years of experience. Across countries, considerable variation was found in the decision to start antihypertensive treatment in the oldest-old ranging from 34 to 88%. In 24/29 (83%) countries, frailty was associated with GPs’ decision not to start treatment even after adjustment for SBP, CVD, and GP characteristics (OR 0.53, 95%CI 0.48–0.59; ORs per country 0.11–1.78).
Conclusions: Across countries, we found considerable variation in starting antihypertensive medication in oldest-old. The frail oldest-old had an odds ratio of 0.53 of receiving antihypertensive treatment. Future hypertension trials should also include frail patients to acquire evidence on the efficacy of antihypertensive treatment in oldest-old patients with frailty, with the aim to get evidence-based data for clinical decision-making.
Non-lethal genotyping of Tribolium castaneum adults using genomic DNA extracted from wing tissue
(2017)
The red flour beetle Tribolium castaneum has become the second most important insect model organism and is frequently used in developmental biology, genetics and pest-associated research. Consequently, the methodological arsenal increases continuously, but many routinely applied techniques for Drosophila melanogaster and other insect species are still unavailable. For example, a protocol for non-lethal genotyping has not yet been adapted but is particularly useful when individuals with known genotypes are required for downstream experiments. In this study, we present a workflow for non-lethal genotyping of T. castaneum adults based on extracting genomic DNA from wing tissue. In detail, we describe a convenient procedure for wing dissection and a custom method for wing digestion that allows PCR-based genotyping of up to fifty adults in less than an afternoon with a success rate of about 86%. The amount of template is sufficient for up to ten reactions while viability and fertility of the beetles are preserved. We prove the applicability of our protocol by genotyping the white / scarlet gene pair alleles from the black-eyed San Bernadino wild-type and white-eyed Pearl recessive mutant strains spanning four generations. Non-lethal genotyping has the potential to improve and accelerate many workflows: Firstly, during the establishment process of homozygous cultures or during stock keeping of cultures that carry recessively lethal alleles, laborious test crossing is replaced by non-lethal genotyping. Secondly, in genome engineering assays, non-lethal genotyping allows the identification of appropriate founders before they are crossed against wild-types, narrowing the efforts down to only the relevant individuals. Thirdly, non-lethal genotyping simplifies experimental strategies, in which genotype and behavior should be correlated, since the genetic configuration of potential individuals can be determined before the actual behavior assays is performed.
Purpose: To evaluate long-term outcome of three years and treatment patterns of patients suffering from severely drug-refractory epilepsy (SDRE).
Methods: This analysis was population-based and retrospective, with data collected from four million individuals insured by statutory German health insurance. ICD-10 codes for epilepsy (G40*) and intake of anticonvulsants were used to identify prevalent cases, which were then compared with a matched cohort drawn from the population at large. Insurance data were available from 2008 to 2013. Any patient who had been prescribed with at least four different antiepileptic drugs (AEDs) in an 18-month period was defined as an SDRE case.
Results: A total of 769 patients with SDRE were identified. Of these, 19% were children and adolescents; the overall mean age was 42.3 years, 45.4% were female and 54.6% male. An average of 2.7 AEDs per patient was prescribed during the first follow-up year. The AEDs most commonly prescribed were: levetiracetam (53.5%), lamotrigine (41.4%), valproate (41.3%), lacosamide (20.4%), and topiramate (17.8%). During 3-year follow-up, there was an annual rate of hospitalization in the range 42.7 to 55%, which was significantly higher than the 11.6–12.8% (p < 0.001) for the matched controls. Admissions to hospital because of epilepsy ranged between 1.7 and 1.9 per year, with an average duration for each epilepsy-caused hospitalization of 10–11.1 days. The number of comorbidities for SDRE patients was significantly increased compared with the matched controls: depression (28% against 10%), vascular disorders (22% against 5%), and injury rates were also higher (head 16% against 3%, trunk and limbs 16% against 8%). The 3-year mortality rate for SDRE patients was 14% against 2.1% in the matched cohort.
Conclusion: SDRE patients are treated with AED polytherapy for all of the 3-year follow-up period. They are hospitalized more frequently than the general population and show increased morbidity levels and a sevenfold increase in mortality rate over 3 years. Further examination is required of ways in which new approaches to treatment could lead to better outcomes in severely affected patients.