The lipid status in patients with ulcerative colitis : Sphingolipids are disease-dependent regulated

  • The factors that contribute to the development of ulcerative colitis (UC), are still not fully identified. Disruption of the colon barrier is one of the first events leading to invasion of bacteria and activation of the immune system. The colon barrier is strongly influenced by sphingolipids. Sphingolipids impact cell–cell contacts and function as second messengers. We collected blood and colon tissue samples from UC patients and healthy controls and investigated the sphingolipids and other lipids by LC-MS/MS or LC-QTOFMS. The expression of enzymes of the sphingolipid pathway were determined by RT-PCR and immunohistochemistry. In inflamed colon tissue, the de novo-synthesis of sphingolipids is reduced, whereas lactosylceramides are increased. Reduction of dihydroceramides was due to posttranslational inhibition rather than altered serine palmitoyl transferase or ceramide synthase expression in inflamed colon tissue. Furthermore, in human plasma from UC-patients, several sphinglipids change significantly in comparison to healthy controls. Beside sphingolipids free fatty acids, lysophosphatidylcholines and triglycerides changed significantly in the blood of colitis patients dependent on the disease severity. Our data indicate that detraction of the sphingolipid de novo synthesis in colon tissue might be an important trigger for UC. Several lipids changed significantly in the blood, which might be used as biomarkers for disease control; however, diet-related variabilities need to be considered.

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Author:Sarah Bazarganipour, Johannes Hausmann, Stephanie Oertel, Khadija El-Hindi, Sebastian Brachtendorf, Irina Ursula BlumensteinORCiDGND, Alica Kubesch, Kathrin Sprinzl, Kerstin Birod, Lisa Hahnefeld, Sandra Trautmann, Dominique Jeanette ThomasORCiDGND, Eva HerrmannORCiD, Gerd GeisslingerORCiDGND, Susanne SchiffmannORCiDGND, Sabine Grösch
Pubmed Id:
Parent Title (English):Journal of Clinical Medicine
Place of publication:Basel
Document Type:Article
Year of Completion:2019
Date of first Publication:2019/07/04
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/09/30
Tag:DHA; EPA; LC–MS/MS; S1P; ceramide; patient; sphingolipid; ulcerative colitis
Issue:7, Art. 971
Page Number:19
First Page:1
Last Page:19
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0