ROS- and radiation source-dependent modulation of leukocyte adhesion to primary microvascular endothelial cells
- Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.
Author: | Denise EckertORCiDGND, Felicitas RappORCiDGND, Ayele Taddese TsedekeGND, Jessica MolendowskaGND, Robert LehnGND, Markus LanghansORCiDGND, Claudia FournierORCiDGND, Franz RödelORCiDGND, Stephanie HehlgansORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-816628 |
DOI: | https://doi.org/10.3390/cells11010072 |
ISSN: | 2073-4409 |
Parent Title (English): | Cells |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/12/27 |
Date of first Publication: | 2021/12/27 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/01/23 |
Tag: | adhesion; endothelial cells; inflammation; leukocytes; low-dose irradiation; shear stress |
Volume: | 11 |
Issue: | 1, art. 72 |
Article Number: | 72 |
Page Number: | 20 |
First Page: | 1 |
Last Page: | 20 |
Note: | This research was funded by the German Federal Ministry of Education and Research (BMBF, grant no. 02NUK050A, 02NUK050D; GREWIS alpha). Results are based on experiments in the context of FAIR Phase-0 at GSI, Darmstadt (Germany). |
Institutes: | Physik |
Medizin | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 53 Physik / 530 Physik |
5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften | |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |