Exploring the application of deep learning for super-resolution microscopy
- Fluorescence microscopy has significantly impacted our understanding of cell biology. The extension of diffraction-unlimited super-resolution microscopy opened an observation window that allows for the scrutiny of cellular organization at a molecular level. The non-invasive nature of visible light in super-resolution microscopy methods renders them suitable for observations in living cells and organisms. Building upon these advancements, a promising synergy between super-resolution fluorescence microscopy and deep learning becomes evident, extending the capabilities of the imaging methods. Tasks such as image modality translation, restoration, single-molecule fitting, virtual labeling, spectral demixing, and molecular counting, are enabled with high precision. The techniques explored in this thesis address three critical facets in advanced microscopy, namely the reduction in image acquisition time, saving photon budget during measurement, and increasing the multiplexing capability. Furthermore, descriptors of protein distributions and their motion on cell membranes were developed.
Author: | Johanna Viola RahmORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-859658 |
DOI: | https://doi.org/10.21248/gups.85965 |
Place of publication: | Frankfurt am Main |
Referee: | Mike HeilemannORCiDGND, Achilleas S. FrangakisORCiDGND |
Document Type: | Doctoral Thesis |
Language: | English |
Date of Publication (online): | 2024/07/10 |
Year of first Publication: | 2024 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Granting Institution: | Johann Wolfgang Goethe-Universität |
Date of final exam: | 2024/06/24 |
Release Date: | 2024/07/10 |
Tag: | deep learning; denoising; receptor tyrosine kinases; super-resolution microscopy; virtual labeling |
Page Number: | 201 |
HeBIS-PPN: | 519720067 |
Institutes: | Biochemie, Chemie und Pharmazie |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |