Biosynthesis of butyrate from methanol and carbon monoxide by recombinant Acetobacterium woodii
- Methanol is one of the most widely produced organic substrates from syngas and can serve as a bio-feedstock to cultivate acetogenic bacteria which allows a major contribution to reducing greenhouse gas. Acetobacterium woodii is one of the very few acetogens that can utilize methanol to produce acetate as sole product. Since A. woodii is genetically tractable, it is an interesting candidate to introduce recombinant pathways for production of bio-commodities from methanol. In this study, we introduced the butyrate production operon from a related acetogen, Eubacterium callanderi KIST612, into A. woodii and show a stable production of butyrate from methanol. This study also reveals how butyrate production by recombinant A. woodii strains can be enhanced with addition of electrons in the form of carbon monoxide. Our results not only show a stable expression system of non-native enzymes in A. woodii but also increase in the product spectrum of A. woodii to compounds with higher economic value.
Author: | Nilanjan Pal ChowdhuryORCiDGND, Dennis LittyGND, Volker MüllerORCiD |
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URN: | urn:nbn:de:hebis:30:3-695729 |
DOI: | https://doi.org/10.1007/s10123-022-00234-z |
ISSN: | 1618-1905 |
Parent Title (English): | International microbiology |
Publisher: | Spanish Soc. for Microbiology |
Place of publication: | Madrid |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2022/02/18 |
Date of first Publication: | 2022/02/18 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/09/06 |
Tag: | Acetogen; Acetogenesis; Bioengineering; Butyrate; Carbon cycling; Wood-Ljungdahl pathway |
Volume: | 25 |
Issue: | 3 |
Page Number: | 10 |
First Page: | 551 |
Last Page: | 560 |
Note: | Open Access funding enabled and organized by Projekt DEAL. The research was funded by an Advanced Grant of the European Research Council under the European Union’s Horizon 2020 Research and Innovation Program (grant agreement no. 741791). |
HeBIS-PPN: | 512572143 |
Institutes: | Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |