Nonribosomal peptides produced by minimal and engineered synthetases with terminal reductase domains
- Nonribosomal peptide synthetases (NRPSs) use terminal reductase domains for 2‐electron reduction of the enzyme‐bound thioester releasing the generated peptides as C‐terminal aldehydes. Herein, we reveal the biosynthesis of a pyrazine that originates from an aldehyde‐generating minimal NRPS termed ATRed in entomopathogenic Xenorhabdus indica. Reductase domains were also investigated in terms of NRPS engineering and, although no general applicable approach was deduced, we show that they can indeed be used for the production of similar natural and unnatural pyrazinones.
Download full text files
Additional Services
| Author: | Andreas Tietze, Yan‐Ni Shi, Max Kronenwerth, Helge Björn BodeORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-565565 |
| DOI: | https://doi.org/10.1002/cbic.202000176 |
| ISSN: | 1439-7633 |
| Parent Title (German): | ChemBioChem |
| Publisher: | Wiley-VCH GmbH |
| Place of publication: | Weinheim |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2020/10/01 |
| Date of first Publication: | 2020/05/07 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2020/11/11 |
| Tag: | NRPS engineering; aldehydes; natural products; nonribosomal peptide synthetases; reductases |
| Volume: | 21 |
| Issue: | 19 |
| Page Number: | 5 |
| First Page: | 2750 |
| Last Page: | 2754 |
| HeBIS-PPN: | 477030858 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
