Asymmetric reconstitution of the erythrocyte anion transport system in vesicles of different curvature: implications for the shape of the band 3 protein
- The anion transport protein of the human erythrocyte membrane, band 3, was solubilized and purified in solutions of the non-ionic detergent nonaethylene glycol lauryl ether and then reconstituted in spherical egg phosphatidylcholine bilayers as described earlier (U. Scheuring, K. Kollewe, W. Haase, and D. Schubert, J. Membrane Biol. 90, 123-135 (1986)). The resulting paucilamellar proteoliposom es of average diameter 70 nm were transformed into smaller vesicles by French press treatment and fractionated according to size by gel filtration. The smallest protein-containing liposomes obtained had diameters around 32 nm; still smaller vesicles were free of protein. All proteoliposome samples studied showed a rapid sulfate efflux which was sensitive to specific inhibitors of band 3-mediated anion exchange. In addition, the orientation of the transport protein in the vesicle membranes was found to be “right-side-out” in all samples. This suggests that the orientation of the protein in the vesicle membranes is dictated by the shape of the protein’s intramembrane domain and that this domain has the form of a truncated cone or pyramid.
Author: | Sabine Lindenthal, Uwe Scheuring, Horst RufGND, Zbigniew Kojro, Winfried Haase, Peter Petrasch, Dieter Schubert |
---|---|
URN: | urn:nbn:de:hebis:30:3-799224 |
DOI: | https://doi.org/10.1515/znc-1990-9-1014 |
ISSN: | 0939-5075 |
ISSN: | 1865-7125 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/2291767 |
Parent Title (German): | Zeitschrift für Naturforschung, C |
Publisher: | Verlag der Zeitschrift für Naturforschung |
Place of publication: | Tübingen |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2014/06/02 |
Year of first Publication: | 1990 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/02/06 |
Tag: | Anion Transport System; Asymmetrie Reconstitution; Band 3 Protein; Erythrocyte Membrane; Protein Shape |
Volume: | 45.1990 |
Issue: | 9-10 |
Page Number: | 6 |
First Page: | 1021 |
Last Page: | 1026 |
Institutes: | Physik |
Biowissenschaften | |
Angeschlossene und kooperierende Institutionen / MPI für Biophysik | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |