Crystallization and X-ray diffraction studies of a complete bacterial fatty-acid synthase type I
- While a deep understanding of the fungal and mammalian multi-enzyme type I fatty-acid synthases (FAS I) has been achieved in recent years, the bacterial FAS I family, which is narrowly distributed within the Actinomycetales genera Mycobacterium, Corynebacterium and Nocardia, is still poorly understood. This is of particular relevance for two reasons: (i) although homologous to fungal FAS I, cryo-electron microscopic studies have shown that bacterial FAS I has unique structural and functional properties, and (ii) M. tuberculosis FAS I is a drug target for the therapeutic treatment of tuberculosis (TB) and therefore is of extraordinary importance as a drug target. Crystals of FAS I from C. efficiens, a homologue of M. tuberculosis FAS I, were produced and diffracted X-rays to about 4.5 Å resolution.
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| Author: | Mathias Enderle, Andrew McCarthyORCiD, Karthik Shivaji PaithankarORCiDGND, Martin GriningerORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-386875 |
| DOI: | https://doi.org/10.1107/S2053230X15018336 |
| ISSN: | 2053-230X |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/26527268 |
| Parent Title (English): | Acta crystallographica / Section F, Structural biology communications |
| Publisher: | Blackwell |
| Place of publication: | Oxford [u.a.] |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2016/11/09 |
| Date of first Publication: | 2015/10/23 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2016/11/09 |
| Tag: | fatty-acid synthase; fatty-acid synthesis; multienzyme; mycolic acid; tuberculosis |
| Volume: | 71 |
| Issue: | Pt 11 |
| Page Number: | 7 |
| First Page: | 1401 |
| Last Page: | 1407 |
| HeBIS-PPN: | 45093022X |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
