Structure of the archaeal chemotaxis protein CheY in a domain-swapped dimeric conformation

  • Archaea are motile by the rotation of the archaellum. The archaellum switches between clockwise and counterclockwise rotation, and movement along a chemical gradient is possible by modulation of the switching frequency. This modulation involves the response regulator CheY and the archaellum adaptor protein CheF. In this study, two new crystal forms and protein structures of CheY are reported. In both crystal forms, CheY is arranged in a domain-swapped conformation. CheF, the protein bridging the chemotaxis signal transduction system and the motility apparatus, was recombinantly expressed, purified and subjected to X-ray data collection.

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Author:Karthik Shivaji PaithankarORCiDGND, Mathias Enderle, David C. Wirthensohn, Arthur Miller, Matthias Schlesner, Friedhelm Pfeiffer, Alexander RittnerORCiDGND, Martin GriningerORCiDGND, Dieter OesterheltGND
URN:urn:nbn:de:hebis:30:3-530826
DOI:https://doi.org/10.1107/S2053230X19010896
ISSN:2053-230X
ISSN:1744-3091
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/31475924
Parent Title (English):Acta crystallographica. Section F, Structural biology communications
Publisher:Blackwell
Place of publication:Oxford [u. a.]
Contributor(s):Norbert Str├Ąter
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/08/30
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2020/03/11
Tag:CheF; CheY; archaellum; chemotaxis; protein evolution; response regulator; signal transduction
Volume:75
Issue:9
Page Number:10
First Page:576
Last Page:585
Note:
This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
HeBIS-PPN:464653266
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universit├Ątspublikationen
Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 4.0