Natural variation in HsfA2 pre‐mRNA splicing is associated with changes in thermotolerance during tomato domestication

  • Summary Wild relatives of crops thrive in habitats where environmental conditions can be restrictive for productivity and survival of cultivated species. The genetic basis of this variability, particularly for tolerance to high temperatures, is not well understood. We examined the capacity of wild and cultivated accessions to acclimate to rapid temperature elevations that cause heat stress (HS). We investigated genotypic variation in thermotolerance of seedlings of wild and cultivated accessions. The contribution of polymorphisms associated with thermotolerance variation was examined regarding alterations in function of the identified gene. We show that tomato germplasm underwent a progressive loss of acclimation to strong temperature elevations. Sensitivity is associated with intronic polymorphisms in the HS transcription factor HsfA2 which affect the splicing efficiency of its pre‐mRNA. Intron splicing in wild species results in increased synthesis of isoform HsfA2‐II, implicated in the early stress response, at the expense of HsfA2‐I which is involved in establishing short‐term acclimation and thermotolerance. We propose that the selection for modern HsfA2 haplotypes reduced the ability of cultivated tomatoes to rapidly acclimate to temperature elevations, but enhanced their short‐term acclimation capacity. Hence, we provide evidence that alternative splicing has a central role in the definition of plant fitness plasticity to stressful conditions.

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Author:Yangjie Hu, Anida Mesihovic, José M. Jiménez‐Gómez, Sascha Oswald Michael Röth, Philipp GebhardtORCiDGND, Daniela Bublak, Arnaud Bovy, Klaus-Dieter Scharf, Enrico SchleiffORCiDGND, Sotirios FragkostefanakisORCiD
Parent Title (English):New phytologist
Place of publication:Oxford [u.a.]
Document Type:Article
Date of Publication (online):2019/09/26
Date of first Publication:2019/09/26
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/10/22
Tag:Solanum; acclimation; high temperature; polymorphism; pre-mRNA splicing; stress response
Page Number:14
First Page:1297
Last Page:1310
Institutes:Biowissenschaften / Biowissenschaften
Wissenschaftliche Zentren und koordinierte Programme / Frankfurt Institute for Advanced Studies (FIAS)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 4.0