Marlene Steiner, Lucas Schneider, Jasmin Yillah, Katharina Gerlach, Olga Nikolaevna Lausen, Annekarin Meyer, Alisa Maring, Halvard-Björn Bönig, Erhard Seifried, Martin Zörnig, Jörn Lausen
- During erythropoiesis, haematopoietic stem cells (HSCs) differentiate in successive steps of commitment and specification to mature erythrocytes. This differentiation process is controlled by transcription factors that establish stage- and cell type-specific gene expression. In this study, we demonstrate that FUSE binding protein 1 (FUBP1), a transcriptional regulator important for HSC self-renewal and survival, is regulated by T-cell acute lymphocytic leukaemia 1 (TAL1) in erythroid progenitor cells. TAL1 directly activates the FUBP1 promoter, leading to increased FUBP1 expression during erythroid differentiation. The binding of TAL1 to the FUBP1 promoter is highly dependent on an intact GATA sequence in a combined E-box/GATA motif. We found that FUBP1 expression is required for efficient erythropoiesis, as FUBP1-deficient progenitor cells were limited in their potential of erythroid differentiation. Thus, the finding of an interconnection between GATA1/TAL1 and FUBP1 reveals a molecular mechanism that is part of the switch from progenitor- to erythrocyte-specific gene expression. In summary, we identified a TAL1/FUBP1 transcriptional relationship, whose physiological function in haematopoiesis is connected to proper erythropoiesis.
MetadatenVerfasserangaben: | Marlene Steiner, Lucas Schneider, Jasmin Yillah, Katharina Gerlach, Olga Nikolaevna Lausen, Annekarin Meyer, Alisa Maring, Halvard-Björn BönigORCiDGND, Erhard SeifriedORCiDGND, Martin ZörnigORCiD, Jörn Lausen |
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URN: | urn:nbn:de:hebis:30:3-486870 |
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DOI: | https://doi.org/10.1371/journal.pone.0210515 |
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ISSN: | 1932-6203 |
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Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/30653565 |
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Titel des übergeordneten Werkes (Englisch): | PLoS one |
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Verlag: | PLoS |
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Verlagsort: | Lawrence, Kan. |
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Sonstige beteiligte Person(en): | Arun Rishi |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Jahr der Fertigstellung: | 2019 |
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Datum der Erstveröffentlichung: | 17.01.2019 |
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Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Datum der Freischaltung: | 22.01.2019 |
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Freies Schlagwort / Tag: | Cell binding; Cell differentiation; Erythropoiesis; Gene expression; Luciferase; Sequence motif analysis; Transcription factors; Transcriptional control |
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Jahrgang: | 14 |
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Ausgabe / Heft: | (1): e0210515 |
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Seitenzahl: | 22 |
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Erste Seite: | 1 |
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Letzte Seite: | 22 |
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Bemerkung: | Copyright: © 2019 Steiner et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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HeBIS-PPN: | 446225347 |
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Institute: | Biochemie, Chemie und Pharmazie / Pharmazie |
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| Biowissenschaften / Biowissenschaften |
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| Medizin / Medizin |
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| Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Open-Access-Publikationsfonds: | Medizin |
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Lizenz (Deutsch): | Creative Commons - Namensnennung 4.0 |
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