Beate Fißlthaler, Nina Zippel, Randa Abdel Malik, Fredy Delgado Lagos, Sven Zukunft, Janina Thoele, Daniel Siuda, Oliver Söhnlein, Ilka Wittig, Juliana Heidler, Andreas Weigert, Ingrid Fleming
- The AMP-activated protein kinase (AMPK) is an energy sensing kinase that is activated by a drop in cellular ATP levels. Although several studies have addressed the role of the AMPKα1 subunit in monocytes and macrophages, little is known about the α2 subunit. The aim of this study was to assess the consequences of AMPKα2 deletion on protein expression in monocytes/macrophages, as well as on atherogenesis. A proteomics approach was applied to bone marrow derived monocytes from wild-type mice versus mice specifically lacking AMPKα2 in myeloid cells (AMPKα2∆MC mice). This revealed differentially expressed proteins, including methyltransferases. Indeed, AMPKα2 deletion in macrophages increased the ratio of S-adenosyl methionine to S-adenosyl homocysteine and increased global DNA cytosine methylation. Also, methylation of the vascular endothelial growth factor and matrix metalloproteinase-9 (MMP9) genes was increased in macrophages from AMPKα2∆MC mice, and correlated with their decreased expression. To link these findings with an in vivo phenotype, AMPKα2∆MC mice were crossed onto the ApoE-/- background and fed a western diet. ApoExAMPKα2∆MC mice developed smaller atherosclerotic plaques than their ApoExα2fl/fl littermates, that contained fewer macrophages and less MMP9 than plaques from ApoExα2fl/fl littermates. These results indicate that the AMPKα2 subunit in myeloid cells influences DNA methylation and thus protein expression and contributes to the development of atherosclerotic plaques.
MetadatenAuthor: | Beate FißlthalerORCiD, Nina Zippel, Randa Abdel Malik, Fredy Delgado Lagos, Sven ZukunftORCiD, Janina Thoele, Daniel Siuda, Oliver Söhnlein, Ilka WittigORCiD, Juliana HeidlerORCiD, Andreas WeigertORCiDGND, Ingrid FlemingORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-510697 |
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DOI: | https://doi.org/10.3390/ijms20123005 |
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ISSN: | 1422-0067 |
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ISSN: | 1661-6596 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/31248224 |
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Parent Title (English): | International journal of molecular sciences |
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Publisher: | Molecular Diversity Preservation International |
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Place of publication: | Basel |
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Document Type: | Article |
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Language: | English |
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Year of Completion: | 2019 |
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Date of first Publication: | 2019/06/19 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2019/09/19 |
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Tag: | DNA methylation; macrophage; matrix metalloproteinase |
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Volume: | 20 |
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Issue: | 12, Art. 3005 |
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Page Number: | 17 |
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First Page: | 1 |
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Last Page: | 17 |
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Note: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
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HeBIS-PPN: | 454035276 |
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Institutes: | Medizin / Medizin |
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| Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Open-Access-Publikationsfonds: | Medizin |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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