Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile
- Shrew-1, also called AJAP1, is a transmembrane protein associated with E-cadherin-mediated adherence junctions and a putative tumor suppressor. Apart from its interaction with β-catenin and involvement in E-cadherin internalization, little structure or function information exists. Here we explored shrew-1 expression during postnatal differentiation of mammary gland as a model system. Immunohistological analyses with antibodies against either the extracellular or the cytoplasmic domains of shrew-1 consistently revealed the expression of full-length shrew-1 in myoepithelial cells, but only part of it in luminal cells. While shrew-1 localization remained unaltered in myoepithelial cells, nuclear localization occurred in luminal cells during lactation. Based on these observations, we identified two unknown shrew-1 transcript variants encoding N-terminally truncated proteins. The smallest shrew-1 protein lacks the extracellular domain and is most likely the only variant present in luminal cells. RNA analyses of human tissues confirmed that the novel transcript variants of shrew-1 exist in vivo and exhibit a differential tissue expression profile. We conclude that our findings are essential for the understanding and interpretation of future functional and interactome analyses of shrew-1 variants.
Author: | Petra Klemmt, Eduard Resch, Isabell Smyrek, Knut Engels, Ernst H. K. StelzerORCiDGND, Anna Starzinski-PowitzORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-451387 |
DOI: | https://doi.org/10.1242/bio.019463 |
ISSN: | 2046-6390 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/27870635 |
Parent Title (English): | Biology open |
Publisher: | Company |
Place of publication: | Cambridge |
Document Type: | Article |
Language: | English |
Year of Completion: | 2016 |
Date of first Publication: | 2016/11/15 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2017/11/30 |
Tag: | AJAP1; Adherence junctions; Lactation; Mammary gland; Splice variants; Tumor suppressor protein |
Volume: | 5 |
Issue: | 11 |
Page Number: | 13 |
First Page: | 1607 |
Last Page: | 1619 |
Note: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed |
HeBIS-PPN: | 427819911 |
Institutes: | Biowissenschaften / Biowissenschaften |
Medizin / Medizin | |
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Biowissenschaften |
Licence (German): | Creative Commons - Namensnennung 3.0 |