Hannah Steinert, Florian Sochor, Anna Wacker, Janina Buck, Christina Helmling, Fabian Hiller, Sara Keyhani, Jonas Noeske, Steffen Grimm, Martin M. Rudolph, Heiko Keller, Rachel Anne Mooney, Rolf van Dick, Beatrix Süß, Boris Fürtig, Jens Wöhnert, Harald Schwalbe
- In bacteria, the regulation of gene expression by cis-acting transcriptional riboswitches located in the 5'-untranslated regions of messenger RNA requires the temporal synchronization of RNA synthesis and ligand binding-dependent conformational refolding. Ligand binding to the aptamer domain of the riboswitch induces premature termination of the mRNA synthesis of ligand-associated genes due to the coupled formation of 3'-structural elements acting as terminators. To date, there has been no high resolution structural description of the concerted process of synthesis and ligand-induced restructuring of the regulatory RNA element. Here, we show that for the guanine-sensing xpt-pbuX riboswitch from Bacillus subtilis, the conformation of the full-length transcripts is static: it exclusively populates the functional off-state but cannot switch to the on-state, regardless of the presence or absence of ligand. We show that only the combined matching of transcription rates and ligand binding enables transcription intermediates to undergo ligand-dependent conformational refolding.
MetadatenAuthor: | Hannah Steinert, Florian Sochor, Anna WackerORCiDGND, Janina BuckGND, Christina Helmling, Fabian HillerGND, Sara KeyhaniGND, Jonas Noeske, Steffen Grimm, Martin M. Rudolph, Heiko Keller, Rachel Anne Mooney, Rolf van DickORCiDGND, Beatrix SüßGND, Boris FürtigORCiDGND, Jens WöhnertORCiDGND, Harald SchwalbeORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-439063 |
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DOI: | https://doi.org/10.7554/eLife.21297.001 |
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ISSN: | 2050-084X |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/28541183 |
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Parent Title (English): | eLife |
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Publisher: | eLife Sciences Publications |
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Place of publication: | Cambridge |
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Contributor(s): | Lewis E. Kay |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2017/10/19 |
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Date of first Publication: | 2017/05/25 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2017/10/19 |
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Tag: | B. subtilis; E. coli; RNA; biochemistry; biophysics; folding; kinetics; meta-stable structures; riboswitches; structural biology; transcription |
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Volume: | 6 |
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Issue: | e21297 |
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Page Number: | 18 |
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First Page: | 1 |
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Last Page: | 18 |
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Note: | Copyright Steinert et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
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HeBIS-PPN: | 424950820 |
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Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
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| Biowissenschaften / Biowissenschaften |
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| Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe |
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| Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs |
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| Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ) |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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