Eva Gruden, Melanie Kienzl, Carina Hasenoehrl, Arailym Sarsembayeva, Dusica Ristic, Sophie Theresa Schmid, Kathrin Maitz, Ulrike Taschler, Lisa Katharina Hahnefeld, Robert Gurke, Dominique Jeanette Thomas, Julia Kargl, Rudolf Schicho
- We recently described that monoacylglycerol lipase (MGL) is present in the tumor microenvironment (TME), increasing tumor growth. In this study we compare the implications of MGL deficiency in the TME in different tumor types.
We show that subcutaneous injection of KP (KrasLSL-G12D/p53fl/fl, mouse lung adenocarcinoma) or B16-F10 cells (mouse melanoma) induced tumor growth in MGL wild type (WT) and knockout (KO) mice. MGL deficiency in the TME attenuated the growth of KP cell tumors whereas tumors from B16-F10 cells increased in size. Opposite immune cell profiles were detected between the two tumor types in MGL KO mice. In line with their anti-tumorigenic function, the number of CD8+ effector T cells and eosinophils increased in KP cell tumors of MGL KO vs. WT mice whereas their presence was reduced in B16-F10 cell tumors of MGL KO mice. Differences were seen in lipid profiles between the investigated tumor types. 2-arachidonoylglycerol (2-AG) content significantly increased in KP, but not B16-F10 cell tumors of MGL KO vs. WT mice while other endocannabinoid-related lipids remained unchanged. However, profiles of phospho- and lysophospholipids, sphingomyelins and fatty acids in KP cell tumors were clearly distinct to those measured in B16-F10 cell tumors.
Our data indicate that TME-localized MGL impacts tumor growth, as well as levels of 2-AG and other lipids in a tumor specific manner.
MetadatenAuthor: | Eva Gruden, Melanie KienzlORCiD, Carina Hasenoehrl, Arailym SarsembayevaORCiD, Dusica RisticORCiD, Sophie Theresa Schmid, Kathrin MaitzORCiD, Ulrike TaschlerORCiD, Lisa Katharina HahnefeldORCiDGND, Robert GurkeORCiDGND, Dominique Jeanette ThomasORCiDGND, Julia KarglORCiD, Rudolf SchichoORCiD |
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URN: | urn:nbn:de:hebis:30:3-771078 |
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DOI: | https://doi.org/10.1016/j.plefa.2023.102585 |
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ISSN: | 0952-3278 |
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Parent Title (English): | Prostaglandins, Leukotrienes and Essential Fatty Acids |
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Publisher: | Elsevier |
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Place of publication: | Amsterdam |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2023/08/11 |
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Date of first Publication: | 2023/08/09 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2023/10/10 |
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Tag: | (lyso)phospholipids; Endocannabinoids; Melanoma; Monoacylglycerol lipase; Non-small cell lung cancer; Tumor microenvironment |
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Volume: | 196 |
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Issue: | 102585 |
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Page Number: | 8 |
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HeBIS-PPN: | 515295663 |
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Institutes: | Medizin |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |
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