• search hit 1 of 161
Back to Result List

Quantitative mass spectrometry-based proteobmics: an overview

  • In recent decades, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass spectrometry has become a focus for further development. In this chapter, we discuss and review actual strategies and problems of the methods for the quantitative analysis of peptides, proteins, and finally proteomes by mass spectrometry. The common themes, the differences, and the potential pitfalls of the main approaches are presented in order to provide a survey of the emerging field of quantitative, mass spectrometry-based proteomics.

Download full text files

Export metadata

Metadaten
Author:Svitlana RozanovaORCiDGND, Katalin BarkovitsORCiDGND, Miroslav Nikolov, Carla SchmidtORCiDGND, Henning UrlaubORCiD, Katrin MarcusGND
URN:urn:nbn:de:hebis:30:3-697677
DOI:https://doi.org/10.1007/978-1-0716-1024-4_8
ISBN:978-1-0716-1023-7
ISBN:978-1-0716-1026-8
ISBN:978-1-0716-1024-4
ISSN:1940-6029
ISSN:1064-3745
Parent Title (English):Quantitative methods in proteomics / edited by Katrin Marcus, Martin Eisenacher, Barbara Sitek ; Methods in molecular biology ; 2228
Publisher:Humana Press ; [Springer]
Place of publication:Totowa, NJ ; [Heidelberg]
Editor:Katrin Marcus, Martin Eisenacher, Barbara Sitek
Document Type:Part of a Book
Language:English
Date of Publication (online):2021/05/05
Date of first Publication:2021/05/05
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/05/06
Tag:Absolute quantification; Chemical labeling; Isotope labeling; Label-free; Mass spectrometry; Metabolic labeling; Proteomics; Relative quantification
Page Number:32
First Page:85
Last Page:116
Note:
The authors were supported by P.U.R.E. (Protein Research Unit Ruhr within Europe) and ProDi (Center for protein diagnostics), Ministry of Innovation, Science and Research of North-Rhine Westphalia, Germany, and by the H2020 project NISCI, (GA no. 681094) and by the ISF program of the European Union.
Institutes:Medizin / Medizin
Angeschlossene und kooperierende Institutionen / MPI für Biophysik
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International

OPUS4 Logo