Unmasking a temperature-dependent effect of the P. anserina i-AAA protease on aging and development
- Different molecular pathways involved in maintaining mitochondrial function are of fundamental importance to control cellular homeostasis. Mitochondrial i-AAA protease is part of such a surveillance system, and PaIAP is the putative ortholog in the fungal aging model Podospora anserina. Here, we investigate the role of PaIAP in aging and development. Deletion of the gene encoding PaIAP resulted in a specific phenotype. When incubated at 27°C, spore germination and fruiting body formation are not different from that of the corresponding wild-type strain. Unexpectedly, the lifespan of the deletion strain is strongly increased. In contrast, cultivation at an elevated temperature of 37°C leads to impairments in spore germination and fruiting body formation and to a reduced lifespan. The higher PaIAP abundance in wild-type strains of the fungus grown at elevated temperature and the phenotype of the deletion strain unmasks a temperature-related role of the protein. The protease appears to be part of a molecular system that has evolved to allow survival under changing temperatures, as they characteristically occur in nature.
Author: | Andrea Weil, Karin Luce, Stefan DröseORCiDGND, Ilka WittigORCiD, Ulrich BrandtORCiDGND, Heinz D. OsiewaczORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-314958 |
DOI: | https://doi.org/10.4161/cc.10.24.18560 |
ISSN: | 1551-4005 |
ISSN: | 1538-4101 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/22134244 |
Parent Title (English): | Cell cycle |
Publisher: | Landes Bioscience |
Place of publication: | Georgetown, Tex. |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2013/09/06 |
Year of first Publication: | 2011 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2013/09/06 |
Tag: | Podospora anserina; aging; i-AAA protease; mitochondria; protein quality control; temperature |
Volume: | 10 |
Issue: | 24 |
Page Number: | 11 |
First Page: | 4280 |
Last Page: | 4290 |
Note: | © 2011 Landes Bioscience |
HeBIS-PPN: | 353136352 |
Institutes: | Biowissenschaften / Biowissenschaften |
Medizin / Medizin | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Sammlung Biologie / Sondersammelgebiets-Volltexte | |
Licence (German): | Deutsches Urheberrecht |