Proteomics of vitreous humor of patients with exudative age-related macular degeneration

  • Background: There is absence of specific biomarkers and an incomplete understanding of the pathophysiology of exudative age-related macular degeneration (AMD). Methods and findings: Eighty-eight vitreous samples (73 from patients with treatment naïve AMD and 15 control samples from patients with idiopathic floaters) were analyzed with capillary electrophoresis coupled to mass spectrometry in this retrospective case series to define potential candidate protein markers of AMD. Nineteen proteins were found to be upregulated in vitreous of AMD patients. Most of the proteins were plasma derived and involved in biological (ion) transport, acute phase inflammatory reaction, and blood coagulation. A number of proteins have not been previously associated to AMD including alpha-1-antitrypsin, fibrinogen alpha chain and prostaglandin H2-D isomerase. Alpha-1-antitrypsin was validated in vitreous of an independent set of AMD patients using Western blot analysis. Further systems biology analysis of the data indicated that the observed proteomic changes may reflect upregulation of immune response and complement activity. Conclusions: Proteome analysis of vitreous samples from patients with AMD, which underwent an intravitreal combination therapy including a core vitrectomy, steroids and bevacizumab, revealed apparent AMD-specific proteomic changes. The identified AMD-associated proteins provide some insight into the pathophysiological changes associated with AMD.

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Author:Michael Janusz Koss, Janosch Hoffmann, Nauke Nguyen, Marcel Pfister, Harald Mischak, William Mullen, Holger Husi, Robert Rejdak, Frank H. Koch, Joachim Jankowski, Katharina Krueger, Thomas Bertelmann, Julie Klein, Joost P. Schanstra, Justyna Siwy
URN:urn:nbn:de:hebis:30:3-337962
DOI:https://doi.org/10.1371/journal.pone.0096895
ISSN:1932-6203
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/24828575
Parent Title (English):PLoS One
Publisher:PLoS
Place of publication:Lawrence, Kan.
Document Type:Article
Language:English
Date of Publication (online):2014/05/14
Date of first Publication:2014/05/14
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2014/05/23
Volume:9
Issue:(5):e96895
Page Number:11
Note:
Copyright: © 2014 Koss et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
HeBIS-PPN:365155934
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0