Vimentin 3, the new hope, differentiating RCC versus oncocytoma

  • Vimentin is currently used to differentiate between malignant renal carcinomas and benign oncocytomas. Recent reports showing Vimentin positive oncocytomas seriously question the validity of this present diagnostic approach. Vimentin 3 is a spliced variant and ends with a unique C-terminal ending after exon 7 which differentiates it from the full length version that has 9 exons. Therefore, the protein size is different; the full length Vimentin version has a protein size of ~57 kDa and the truncated version of ~47 kDa. We designed an antibody, called Vim3, against the unique C-terminal ending of the Vimentin 3 variant. Using immune histology, immune fluorescence, Western blot, and qRT-PCR analysis, a Vim3 overexpression was detectable exclusively in oncocytoma, making the detection of Vim3 a potential specific marker for benign kidney tumors. This antibody is the first to clearly differentiate benign oncocytoma and the mimicking eosinophilic variants of the RCCs. This differentiation between malignant and benign RCCs is essential for operative planning, follow-up therapy, and patients' survival. In the future the usage of Vimentin antibodies in routine pathology has to be applied with care. Consideration must be given to Vimentin specific binding epitopes otherwise a misdiagnosis of the patients' tumor samples may result.

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Author:Melanie von Brandenstein, Katharina Christine Pütz, Barbara Monika Schlosser, Heike Löser, Joachim Peter Kallinowski, Daniel Gödde, Reinhard Büttner, Stefan Störkel, Jochen W. U. Fries
URN:urn:nbn:de:hebis:30:3-423822
DOI:https://doi.org/10.1155/2015/368534
ISSN:1875-8630
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/25944973
Parent Title (English):Disease markers
Publisher:Hindawi
Place of publication:New York, NY [u.a.]
Document Type:Article
Language:English
Date of Publication (online):2016/12/08
Date of first Publication:2015/04/07
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2016/12/08
Volume:2015
Issue:Article ID 368534
Page Number:8
Note:
Copyright © 2015 Melanie von Brandenstein  et al. This is an open access article distributed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HeBIS-PPN:428597580
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 3.0