Suppression of the interleukin-1ß-induced inflammatory response of human Chang liver cells by acute and subacute exposure to alcohol : an in vitro study

Suzbija li akutna ili subakutna izloženost alkoholu upalni odgovor Chang stanica jetre izazvan interleukinom-1β? : in vitro istraživanje

  • Aim: To evaluate protective immunosuppressive dose and time-dependent effects of ethanol in an in vitro model of acute inflammation in human Chang liver cells. Method: The study was performed in 2016 and 2017 in the research laboratory of the Department of Trauma, Hand and Reconstructive Surgery, the University Hospital of the Goethe-University Frankfurt. Chang liver cells were stimu - lated with either interleukin (IL)-1β or IL-6 and subsequent - ly treated with low-dose ethanol (85 mmol/L) or high-dose ethanol (170 mmol/L) for one hour (acute exposure) or 72 hours (subacute exposure). IL-6 and IL-1β release were de - termined by enzyme-linked immunosorbent assay. Neu - trophil adhesion to Chang liver monolayers, production of reactive oxygen species, and apoptosis or necrosis were analyzed. Results: Contrary to high-dose ethanol, acute low-dose ethanol exposure significantly reduced IL-1β-induced IL-6 and IL-6-induced IL-1β release ( P <0.05). Subacute etha - nol exposure did not change proinflammatory cytokine release. Acute low-dose ethanol exposure significantly decreased inflammation-induced formation of reactive oxygen species ( P <0.05) and significantly improved cell survival ( P <0.05). Neither acute nor subacute high-dose ethanol exposure significantly changed inflammationinduced changes in reactive oxygen species or survival. Acute and subacute ethanol exposure, independently of the dose, significantly decreased neutrophil adhesion to inflamed Chang liver cells ( P <0.05). Conclusion: Acute treatment of inflamed Chang liver cells with ethanol showed its immunosuppressive potential. However, the observed effects were limited to low-dose setting, indicating the relevance of ethanol dose in the modulation of inflammatory cell response.
  • Cilj: Odrediti protektivne imunosupresijske učinke etanola ovisne o dozi i vremenu u in vitro modelu akutne upale u ljudskim Chang stanicama jetre. Postupci: Istraživanje je provedeno 2016. i 2017. u istraživačkom laboratoriju Zavoda za traumatologiju, kirurgiju ruke i rekonstruktivnu kirurgiju Bolnice Sveučilišta Goethe u Frankfurtu. Chang stanice jetre stimulirane su ili interleukinom-1β ili interleukinom-6 te tretirane niskom (85 mmol/L) ili visokom dozom etanola (170 mmol/L) jedan sat (akutna izloženost) ili 72 sata (subakutna izloženost). Otpuštanje IL-6 i IL-1β određeno je enzimskim imunotestom. Također smo analizirali adheziju neutrofila na monoslojeve Chang stanica jetre, proizvodnju reaktivnih vrsta kisika i apoptozu ili nekrozu. Rezultati: Nasuprot visokoj dozi, niska doza etanola značajno je smanjila otpuštanje interleukina-6 izazvano interleukinom-1β i otpuštanje interleukina-1β izazvano interleukinom-6 (P < 0,05). Subakutna izloženost etanolu nije promijenila proinflamatorno otpuštanje citokina. Akutna niska doza etanola značajno je smanjila formiranje reaktivnih vrsta kisika izazvano upalom (P < 0.05) i značajno je poboljšala preživljenje stanica (P < 0.05). Ni akutna ni subakutna visoka doza etanola nisu značajno utjecale na promjene izazvane upalom kod reaktivnih vrsta kisika i preživljenja stanica. Akutna i subakutna doza etanola, neovisno o dozi, značajno su smanjile adheziju neutrofila na upaljene Chang stanice jetre (P < 0.05). Zaključak: Akutna izloženost Chang stanica jetre etanolu pokazala je njegov imunosupresivni potencijal. Međutim, takvi učinci zabilježeni su samo za nisku dozu, upućujući na važnost doze etanola u modulaciji upalnog odgovora stanica.

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Author:Katharina Mörs, Shinwan Kany, Jason-Alexander Hörauf, Nils Wagner, Claudia NeunaberORCiDGND, Mario Perl, Ingo MarziORCiDGND, Borna ReljaORCiDGND
URN:urn:nbn:de:hebis:30:3-466930
DOI:https://doi.org/10.3325/cmj.2018.59.46
ISSN:1332-8166
ISSN:0353-9504
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/29740988
Parent Title (English):Croatian medical journal
Publisher:Univ. of Zagreb, School of Medicine
Place of publication:Zagreb
Document Type:Article
Language:English
Year of Completion:2018
Date of first Publication:2018/04/30
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/09/20
Volume:59
Issue:2
Page Number:10
First Page:46
Last Page:55
Note:
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
HeBIS-PPN:437851117
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0