Separating mitochondrial protein assembly and endoplasmic reticulum tethering by selective coupling of Mdm10

  • The endoplasmic reticulum–mitochondria encounter structure (ERMES) connects the mitochondrial outer membrane with the ER. Multiple functions have been linked to ERMES, including maintenance of mitochondrial morphology, protein assembly and phospholipid homeostasis. Since the mitochondrial distribution and morphology protein Mdm10 is present in both ERMES and the mitochondrial sorting and assembly machinery (SAM), it is unknown how the ERMES functions are connected on a molecular level. Here we report that conserved surface areas on opposite sides of the Mdm10 β-barrel interact with SAM and ERMES, respectively. We generated point mutants to separate protein assembly (SAM) from morphology and phospholipid homeostasis (ERMES). Our study reveals that the β-barrel channel of Mdm10 serves different functions. Mdm10 promotes the biogenesis of α-helical and β-barrel proteins at SAM and functions as integral membrane anchor of ERMES, demonstrating that SAM-mediated protein assembly is distinct from ER-mitochondria contact sites.

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Author:Lars Ellenrieder, Łukasz Opaliński, Lars Becker, Vivien Krüger, Oliver MirusGND, Sebastian Philipp Straub, Katharina Ebell, Nadine FlinnerORCiDGND, Sebastian Stiller, Bernard Guiard, Chris Meisinger, Nils Wiedemann, Enrico SchleiffORCiDGND, Richard Wagner, Nikolaus Pfanner, Thomas Becker
Pubmed Id:
Parent Title (English):Nature Communications
Publisher:Nature Publishing Group UK
Place of publication:[London]
Document Type:Article
Year of Completion:2016
Date of first Publication:2016/10/10
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/11/22
Tag:Mitochondria; Proteins
Issue:Art. 13021
Page Number:14
First Page:1
Last Page:14
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Institutes:Biowissenschaften / Biowissenschaften
Informatik und Mathematik / Informatik
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Wissenschaftliche Zentren und koordinierte Programme / Frankfurt Institute for Advanced Studies (FIAS)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0